The human airway epithelial basal cell transcriptome

Neil R. Hackett, Renat Shaykhiev, Matthew S. Walters, Rui Wang, Rachel K. Zwick, Barbara Ferris, Bradley Witover, Jacqueline Salit, Ronald Crystal

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

Background: The human airway epithelium consists of 4 major cell types: ciliated, secretory, columnar and basal cells. During natural turnover and in response to injury, the airway basal cells function as stem/progenitor cells for the other airway cell types. The objective of this study is to better understand human airway epithelial basal cell biology by defining the gene expression signature of this cell population. Methodology/Principal Findings: Bronchial brushing was used to obtain airway epithelium from healthy nonsmokers. Microarrays were used to assess the transcriptome of basal cells purified from the airway epithelium in comparison to the transcriptome of the differentiated airway epithelium. This analysis identified the "human airway basal cell signature" as 1,161 unique genes with >5-fold higher expression level in basal cells compared to differentiated epithelium. The basal cell signature was suppressed when the basal cells differentiated into a ciliated airway epithelium in vitro. The basal cell signature displayed overlap with genes expressed in basal-like cells from other human tissues and with that of murine airway basal cells. Consistent with self-modulation as well as signaling to other airway cell types, the human airway basal cell signature was characterized by genes encoding extracellular matrix components, growth factors and growth factor receptors, including genes related to the EGF and VEGF pathways. Interestingly, while the basal cell signature overlaps that of basal-like cells of other organs, the human airway basal cell signature has features not previously associated with this cell type, including a unique pattern of genes encoding extracellular matrix components, G protein-coupled receptors, neuroactive ligands and receptors, and ion channels. Conclusion/Significance: The human airway epithelial basal cell signature identified in the present study provides novel insights into the molecular phenotype and biology of the stem/progenitor cells of the human airway epithelium.

Original languageEnglish
Article numbere18378
JournalPLoS One
Volume6
Issue number5
DOIs
Publication statusPublished - 13 May 2011
Externally publishedYes

Fingerprint

Transcriptome
transcriptome
Epithelial Cells
Cells
cells
Epithelium
epithelium
stem cells
Stem Cells
Gene encoding
Genes
Stem cells
genes
extracellular matrix
Cytology
Extracellular Matrix
growth factors
Growth Factor Receptors
Microarrays
G-Protein-Coupled Receptors

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Hackett, N. R., Shaykhiev, R., Walters, M. S., Wang, R., Zwick, R. K., Ferris, B., ... Crystal, R. (2011). The human airway epithelial basal cell transcriptome. PLoS One, 6(5), [e18378]. https://doi.org/10.1371/journal.pone.0018378

The human airway epithelial basal cell transcriptome. / Hackett, Neil R.; Shaykhiev, Renat; Walters, Matthew S.; Wang, Rui; Zwick, Rachel K.; Ferris, Barbara; Witover, Bradley; Salit, Jacqueline; Crystal, Ronald.

In: PLoS One, Vol. 6, No. 5, e18378, 13.05.2011.

Research output: Contribution to journalArticle

Hackett, NR, Shaykhiev, R, Walters, MS, Wang, R, Zwick, RK, Ferris, B, Witover, B, Salit, J & Crystal, R 2011, 'The human airway epithelial basal cell transcriptome', PLoS One, vol. 6, no. 5, e18378. https://doi.org/10.1371/journal.pone.0018378
Hackett NR, Shaykhiev R, Walters MS, Wang R, Zwick RK, Ferris B et al. The human airway epithelial basal cell transcriptome. PLoS One. 2011 May 13;6(5). e18378. https://doi.org/10.1371/journal.pone.0018378
Hackett, Neil R. ; Shaykhiev, Renat ; Walters, Matthew S. ; Wang, Rui ; Zwick, Rachel K. ; Ferris, Barbara ; Witover, Bradley ; Salit, Jacqueline ; Crystal, Ronald. / The human airway epithelial basal cell transcriptome. In: PLoS One. 2011 ; Vol. 6, No. 5.
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