The globular domain of histone H5 is internally located in the 30 nm chromatin fiber

an immunochemical study.

S. I. Dimitrov, V. R. Russanova, I. G. Pashev

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The location of the globular domain of histone H5 relative to the axis of the 30 nm chromatin fiber was investigated by following the accessibility of this region of the molecule in chicken erythrocyte chromatin to specific antibodies as a function of chromatin structure. Antibodies to the globular domain of H5 as well as their Fab fragments were found to react with chromatin at ionic strengths ranging from 1-80 mM NaCl, the reaction gradually decreasing upon increase of salt concentration. If, however, Fab fragments were conjugated to ferritin, no reaction of the complex with chromatin was observed at salt concentrations higher than 20 mM. The accessibility of the globular part of H5 in unfolded chromatin to the Fab-ferritin complex was also demonstrated with trypsin-digested chromatin. The experiments were carried out by both solid-phase immunoassay and inhibition experiments. The data obtained are consistent with a structure in which the globular domain of H5 is internally located in the 30 nm chromatin fiber.

Original languageEnglish
Pages (from-to)2387-2392
Number of pages6
JournalEMBO Journal
Volume6
Issue number8
Publication statusPublished - Aug 1987
Externally publishedYes

Fingerprint

Histones
Chromatin
Fibers
Immunoglobulin Fab Fragments
Ferritins
Salts
Antibodies
Ionic strength
Immunoassay
Osmolar Concentration
Trypsin
Chickens
Erythrocytes
Experiments
Molecules

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

The globular domain of histone H5 is internally located in the 30 nm chromatin fiber : an immunochemical study. / Dimitrov, S. I.; Russanova, V. R.; Pashev, I. G.

In: EMBO Journal, Vol. 6, No. 8, 08.1987, p. 2387-2392.

Research output: Contribution to journalArticle

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