The Elav-like protein HuR exerts translational control of viral internal ribosome entry sites

Andrea Rivas-Aravena, Pablo Ramdohr, Maricarmen Vallejos, Fernando Valiente-Echeverría, Virginie Dormoy-Raclet, Felipe Rodríguez, Karla Pino, Cristian Holzmann, J. Pablo Huidobro-Toro, Imed Eddine Gallouzi, Marcelo López-Lastra

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Abstract

The human embryonic-lethal abnormal vision (ELAV)-like protein, HuR, has been recently found to be involved in the regulation of protein synthesis. In this study we show that HuR participates in the translational control of the HIV-1 and HCV IRES elements. HuR functions as a repressor of HIV-1 IRES activity and acts as an activator of the HCV IRES. The effect of HuR was evaluated in three independent experimental systems, rabbit reticulocyte lysate, HeLa cells, and Xenopus laevis oocytes, using both overexpression and knockdown approaches. Furthermore, results suggest that HuR mediated regulation of HIV-1 and HCV IRESes does not require direct binding of the protein to the RNA nor does it need the nuclear translocation of the IRES-containing RNAs. Finally, we show that HuR has a negative impact on post-integration steps of the HIV-1 replication cycle. Thus, our observations yield novel insights into the role of HuR in the post-transcriptional regulation of HCV and HIV-1 gene expression.

Original languageEnglish
Pages (from-to)178-185
Number of pages8
JournalVirology
Volume392
Issue number2
DOIs
Publication statusPublished - 30 Sep 2009

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Keywords

  • HCV
  • HIV-1
  • HuR
  • IRES
  • ITAF
  • Translation initiation

ASJC Scopus subject areas

  • Virology

Cite this

Rivas-Aravena, A., Ramdohr, P., Vallejos, M., Valiente-Echeverría, F., Dormoy-Raclet, V., Rodríguez, F., Pino, K., Holzmann, C., Huidobro-Toro, J. P., Gallouzi, I. E., & López-Lastra, M. (2009). The Elav-like protein HuR exerts translational control of viral internal ribosome entry sites. Virology, 392(2), 178-185. https://doi.org/10.1016/j.virol.2009.06.050