The effects of removing external sodium upon the control of potassium (86Rb+) permeability in the isolated human sweat gland

SM Wilson, Douglas Bovell, HY Elder, DM Jenkinson, JD Pediani

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The changes in cytoplasmic free calcium ([Ca2+]i) which occur in isolated human sweat glands during cholinergic stimulation have been studied indirectly by monitoring potassium permeability. The acetylcholine‐evoked permeability increase normally consists of transient and sustained phases which are attributed to the mobilization of intracellular calcium stores and to calcium influx respectively. Such consistent responses to acetylcholine could not be obtained during superfusion with bicarbonate‐free, HEPES‐buffered solutions. The human sweat gland in vitro therefore appears to have a strict requirement for bicarbonate. The sustained component of the response was not affected by total removal of external sodium, suggesting that calcium influx does not occur via a sodium‐dependent system. The transient component, however, was abolished when external sodium was replaced by N‐methyl‐D‐glucammonium (NMDG+). It therefore appears that secretagogue‐evoked mobilization of cytoplasmic calcium is dependent, in some way, upon external sodium. This dependence is not, however, absolute as the response was essentially normal when sodium was replaced by lithium.

Original languageEnglish
Pages (from-to)649-656
Number of pages8
JournalExperimental Physiology
Volume75
Issue number5
DOIs
Publication statusPublished - 1 Sep 1990
Externally publishedYes

Fingerprint

Sweat Glands
Permeability
Potassium
Sodium
Calcium
Bicarbonates
Lithium
Cholinergic Agents
Acetylcholine

ASJC Scopus subject areas

  • Physiology

Cite this

The effects of removing external sodium upon the control of potassium (86Rb+) permeability in the isolated human sweat gland. / Wilson, SM; Bovell, Douglas; Elder, HY; Jenkinson, DM; Pediani, JD.

In: Experimental Physiology, Vol. 75, No. 5, 01.09.1990, p. 649-656.

Research output: Contribution to journalArticle

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