The effects of centrally administered apelin-13 on food intake, water intake and pituitary hormone release in rats

Shahrad Taheri, Kevin Murphy, Mark Cohen, Elizabeth Sujkovic, Adam Kennedy, Waljit Dhillo, Catherine Dakin, Arshia Sajedi, Mohammad Ghatei, Stephen Bloom

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232 Citations (Scopus)

Abstract

Apelin is the recently identified endogenous ligand for the G-protein-coupled receptor, APJ. Preproapelin and APJ mRNA are found in hypothalamic regions known to be important in the regulation of food and water intake, and pituitary hormone release. The effects of intracerebroventricular (ICV) administration of pyroglutamylated apelin-13 on food and water intake and pituitary hormone release in rats were investigated. Apelin-13 had little effect on food intake, but dose-dependently increased drinking behaviour and water intake at 1 h. Apelin-13 (10 nmol) increased water intake by up to sixfold compared to saline. Compared to saline control, apelin-13 (10 nmol) significantly increased plasma ACTH and corticosterone and decreased plasma prolactin, LH and FSH at 30 min. In vitro, apelin-13 stimulated the release of CRH and AVP from hypothalamic explants, but had no effect on NPY release. These results suggest that apelin may play an important role in the hypothalamic regulation of water intake and endocrine axes.

Original languageEnglish
Pages (from-to)1208-1212
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume291
Issue number5
DOIs
Publication statusPublished - 2002
Externally publishedYes

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Keywords

  • Apelin
  • APJ receptor
  • Food intake
  • Hypothalamus
  • Pituitary hormones
  • Water intake

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Taheri, S., Murphy, K., Cohen, M., Sujkovic, E., Kennedy, A., Dhillo, W., Dakin, C., Sajedi, A., Ghatei, M., & Bloom, S. (2002). The effects of centrally administered apelin-13 on food intake, water intake and pituitary hormone release in rats. Biochemical and Biophysical Research Communications, 291(5), 1208-1212. https://doi.org/10.1006/bbrc.2002.6575