The dynamics of individual nucleosomes controls the chromatin condensation pathway

Direct atomic force microscopy visualization of variant chromatin

Fabien Montel, Hervé Menoni, Martin Castelnovo, Jan Bednar, Stefan Dimitrov, Dimitar Angelov, Cendrine Faivre-Moskalenko

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Chromatin organization and dynamics is studied at scales ranging from single nucleosome to nucleosomal array by using a unique combination of biochemical assays, single molecule imaging technique, and numerical modeling. We show that a subtle modification in the nucleosome structure induced by the histone variant H2A.Bbd drastically modifies the higher order organization of the nucleosomal arrays. Importantly, as directly visualized by atomic force microscopy, conventional H2A nucleosomal arrays exhibit specific local organization, in contrast to H2A.Bbd arrays, which show "beads on a string" structure. The combination of systematic image analysis and theoretical modeling allows a quantitative description relating the observed gross structural changes of the arrays to their local organization. Our results suggest strongly that higher-order organization of H1-free nucleosomal arrays is determined mainly by the fluctuation properties of individual nucleosomes. Moreover, numerical simulations suggest the existence of attractive interactions between nucleosomes to provide the degree of compaction observed for conventional chromatin fibers.

Original languageEnglish
Pages (from-to)544-553
Number of pages10
JournalBiophysical Journal
Volume97
Issue number2
DOIs
Publication statusPublished - 2009
Externally publishedYes

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Nucleosomes
Atomic Force Microscopy
Chromatin
Histones

ASJC Scopus subject areas

  • Biophysics

Cite this

The dynamics of individual nucleosomes controls the chromatin condensation pathway : Direct atomic force microscopy visualization of variant chromatin. / Montel, Fabien; Menoni, Hervé; Castelnovo, Martin; Bednar, Jan; Dimitrov, Stefan; Angelov, Dimitar; Faivre-Moskalenko, Cendrine.

In: Biophysical Journal, Vol. 97, No. 2, 2009, p. 544-553.

Research output: Contribution to journalArticle

Montel, Fabien ; Menoni, Hervé ; Castelnovo, Martin ; Bednar, Jan ; Dimitrov, Stefan ; Angelov, Dimitar ; Faivre-Moskalenko, Cendrine. / The dynamics of individual nucleosomes controls the chromatin condensation pathway : Direct atomic force microscopy visualization of variant chromatin. In: Biophysical Journal. 2009 ; Vol. 97, No. 2. pp. 544-553.
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