The cystic fibrosis gene has a ''housekeeping''-type promoter and is expressed at low levels in cells of epithelial origin

K. Yoshimura, H. Nakamura, B. C. Trapnell, W. Dalemans, A. Pavirani, J. P. Lecocq, Ronald Crystal

Research output: Contribution to journalArticle

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Abstract

Evaluation of the expression of the cystic fibrosis (CF) gene in human epithelial cell lines demonstrated active, but low level, transcription of the gene. Analysis of 3.8 kilobases of genomic sequences 5' to exon 1 of the CF gene demonstrated no TATA promoter element, but a high G + C content, multiple transcription start sites, and several potential Sp1 binding sites. Fragments of 5'-flanking sequences from 2.2 kilobases to as small as 102 base pairs 5' to the major transcription start site supported constitutive reporter gene expression in epithelial cells, but at low levels, and independent of the length of the 5' fragment. CF gene transcription was down-regulated by phorbol myristate acetate. Importantly, evaluation of freshly isolated normal human bronchial cells also demonstrated CF gene transcription at a relatively low rate. Together, these observations suggest that although the normal CF gene promoter has characteristics of a ''housekeeping''-type gene, and the gene is expressed at low levels in cells of organs that manifest the clinical disorder ''cystic fibrosis,'' its expression can be modulated transcriptionally, implying a possible therapeutic approach for the disease.

Original languageEnglish
Pages (from-to)9140-9144
Number of pages5
JournalJournal of Biological Chemistry
Volume266
Issue number14
Publication statusPublished - 22 Jul 1991
Externally publishedYes

Fingerprint

Housekeeping
Cystic Fibrosis
Genes
Epithelial Cells
Transcription
Transcription Initiation Site
5' Flanking Region
Essential Genes
Base Composition
Tetradecanoylphorbol Acetate
Reporter Genes
Base Pairing
Exons
Gene expression
Binding Sites
Gene Expression
Cell Line

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Yoshimura, K., Nakamura, H., Trapnell, B. C., Dalemans, W., Pavirani, A., Lecocq, J. P., & Crystal, R. (1991). The cystic fibrosis gene has a ''housekeeping''-type promoter and is expressed at low levels in cells of epithelial origin. Journal of Biological Chemistry, 266(14), 9140-9144.

The cystic fibrosis gene has a ''housekeeping''-type promoter and is expressed at low levels in cells of epithelial origin. / Yoshimura, K.; Nakamura, H.; Trapnell, B. C.; Dalemans, W.; Pavirani, A.; Lecocq, J. P.; Crystal, Ronald.

In: Journal of Biological Chemistry, Vol. 266, No. 14, 22.07.1991, p. 9140-9144.

Research output: Contribution to journalArticle

Yoshimura, K, Nakamura, H, Trapnell, BC, Dalemans, W, Pavirani, A, Lecocq, JP & Crystal, R 1991, 'The cystic fibrosis gene has a ''housekeeping''-type promoter and is expressed at low levels in cells of epithelial origin', Journal of Biological Chemistry, vol. 266, no. 14, pp. 9140-9144.
Yoshimura K, Nakamura H, Trapnell BC, Dalemans W, Pavirani A, Lecocq JP et al. The cystic fibrosis gene has a ''housekeeping''-type promoter and is expressed at low levels in cells of epithelial origin. Journal of Biological Chemistry. 1991 Jul 22;266(14):9140-9144.
Yoshimura, K. ; Nakamura, H. ; Trapnell, B. C. ; Dalemans, W. ; Pavirani, A. ; Lecocq, J. P. ; Crystal, Ronald. / The cystic fibrosis gene has a ''housekeeping''-type promoter and is expressed at low levels in cells of epithelial origin. In: Journal of Biological Chemistry. 1991 ; Vol. 266, No. 14. pp. 9140-9144.
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