The complete genome sequence and analysis of the epsilonproteobacterium Arcobacter butzleri

William G. Miller, Craig T. Parker, Marc Rubenfield, George L. Mendz, Marc M.S.M. Wösten, David W. Ussery, John F. Stolz, Tim T. Binnewies, Peter F. Hallin, Guilin Wang, Joel Malek, Andrea Rogosin, Larry H. Stanker, Robert E. Mandrell

Research output: Contribution to journalArticle

130 Citations (Scopus)

Abstract

Background. Arcobacter butzleri is a member of the epsilon subdivision of the Proteobacteria and a close taxonomic relative of established pathogens, such as Campylobacter jejuni and Helicobacter pylori. Here we present the complete genome sequence of the human clinical isolate, A. butzleri strain RM4018. Methodology/Principal Findings. Arcobacter butzleri is a member of the Campylobacteraceae, but the majority of its proteome is most similar to those of Sulfuromonas denitrificans and Wolinella succinogenes, both members of the Helicobacteraceae, and those of the deep-sea vent Epsilonproteobacteria Sulfurovum and Nitratiruptor. In addition, many of the genes and pathways described here, e.g. those involved in signal transduction and sulfur metabolism, have been identified previously within the epsilon subdivision only in S. denitrificans, W. succinogenes, Sulfurovum, and/or Nitratiruptor, or are unique to the subdivision. In addition, the analyses indicated also that a substantial proportion of the A. butzleri genome is devoted to growth and survival under diverse environmental conditions, with a large number of respiration-associated proteins, signal transduction and chemotaxis proteins and proteins involved in DNA repair and adaptation. To investigate the genomic diversity of A. butzleri strains, we constructed an A. butzleri DNA microarray comprising 2238 genes from strain RM4018. Comparative genomic indexing analysis of 12 additional A. butzleri strains identified both the core genes of A. butzleri and intraspecies hypervariable regions, where <70% of the genes were present in at least two strains. Conclusion/Significance. The presence of pathways and loci associated often with non-host-associated organisms, as well as genes associated with virulence, suggests that A. butzleri is a free-living, water-borne organism that might be classified rightfully as an emerging pathogen. The genome sequence and analyses presented in this study are an important first step in understanding the physiology and genetics of this organism, which constitutes a bridge between the environment and mammalian hosts.

Original languageEnglish
Article numbere1358
JournalPLoS One
Volume2
Issue number12
DOIs
Publication statusPublished - 26 Dec 2007
Externally publishedYes

Fingerprint

Epsilonproteobacteria
Arcobacter
Arcobacter butzleri
epsilon-Proteobacteria
Sequence Analysis
Genes
Genome
genome
Wolinella succinogenes
Wolinella
Signal transduction
Pathogens
genes
Helicobacter pylori
Helicobacteraceae
signal transduction
Campylobacteraceae
Signal Transduction
organisms
Hydrothermal Vents

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Miller, W. G., Parker, C. T., Rubenfield, M., Mendz, G. L., Wösten, M. M. S. M., Ussery, D. W., ... Mandrell, R. E. (2007). The complete genome sequence and analysis of the epsilonproteobacterium Arcobacter butzleri. PLoS One, 2(12), [e1358]. https://doi.org/10.1371/journal.pone.0001358

The complete genome sequence and analysis of the epsilonproteobacterium Arcobacter butzleri. / Miller, William G.; Parker, Craig T.; Rubenfield, Marc; Mendz, George L.; Wösten, Marc M.S.M.; Ussery, David W.; Stolz, John F.; Binnewies, Tim T.; Hallin, Peter F.; Wang, Guilin; Malek, Joel; Rogosin, Andrea; Stanker, Larry H.; Mandrell, Robert E.

In: PLoS One, Vol. 2, No. 12, e1358, 26.12.2007.

Research output: Contribution to journalArticle

Miller, WG, Parker, CT, Rubenfield, M, Mendz, GL, Wösten, MMSM, Ussery, DW, Stolz, JF, Binnewies, TT, Hallin, PF, Wang, G, Malek, J, Rogosin, A, Stanker, LH & Mandrell, RE 2007, 'The complete genome sequence and analysis of the epsilonproteobacterium Arcobacter butzleri', PLoS One, vol. 2, no. 12, e1358. https://doi.org/10.1371/journal.pone.0001358
Miller WG, Parker CT, Rubenfield M, Mendz GL, Wösten MMSM, Ussery DW et al. The complete genome sequence and analysis of the epsilonproteobacterium Arcobacter butzleri. PLoS One. 2007 Dec 26;2(12). e1358. https://doi.org/10.1371/journal.pone.0001358
Miller, William G. ; Parker, Craig T. ; Rubenfield, Marc ; Mendz, George L. ; Wösten, Marc M.S.M. ; Ussery, David W. ; Stolz, John F. ; Binnewies, Tim T. ; Hallin, Peter F. ; Wang, Guilin ; Malek, Joel ; Rogosin, Andrea ; Stanker, Larry H. ; Mandrell, Robert E. / The complete genome sequence and analysis of the epsilonproteobacterium Arcobacter butzleri. In: PLoS One. 2007 ; Vol. 2, No. 12.
@article{be6a716058ce477897ea480dda15da0a,
title = "The complete genome sequence and analysis of the epsilonproteobacterium Arcobacter butzleri",
abstract = "Background. Arcobacter butzleri is a member of the epsilon subdivision of the Proteobacteria and a close taxonomic relative of established pathogens, such as Campylobacter jejuni and Helicobacter pylori. Here we present the complete genome sequence of the human clinical isolate, A. butzleri strain RM4018. Methodology/Principal Findings. Arcobacter butzleri is a member of the Campylobacteraceae, but the majority of its proteome is most similar to those of Sulfuromonas denitrificans and Wolinella succinogenes, both members of the Helicobacteraceae, and those of the deep-sea vent Epsilonproteobacteria Sulfurovum and Nitratiruptor. In addition, many of the genes and pathways described here, e.g. those involved in signal transduction and sulfur metabolism, have been identified previously within the epsilon subdivision only in S. denitrificans, W. succinogenes, Sulfurovum, and/or Nitratiruptor, or are unique to the subdivision. In addition, the analyses indicated also that a substantial proportion of the A. butzleri genome is devoted to growth and survival under diverse environmental conditions, with a large number of respiration-associated proteins, signal transduction and chemotaxis proteins and proteins involved in DNA repair and adaptation. To investigate the genomic diversity of A. butzleri strains, we constructed an A. butzleri DNA microarray comprising 2238 genes from strain RM4018. Comparative genomic indexing analysis of 12 additional A. butzleri strains identified both the core genes of A. butzleri and intraspecies hypervariable regions, where <70{\%} of the genes were present in at least two strains. Conclusion/Significance. The presence of pathways and loci associated often with non-host-associated organisms, as well as genes associated with virulence, suggests that A. butzleri is a free-living, water-borne organism that might be classified rightfully as an emerging pathogen. The genome sequence and analyses presented in this study are an important first step in understanding the physiology and genetics of this organism, which constitutes a bridge between the environment and mammalian hosts.",
author = "Miller, {William G.} and Parker, {Craig T.} and Marc Rubenfield and Mendz, {George L.} and W{\"o}sten, {Marc M.S.M.} and Ussery, {David W.} and Stolz, {John F.} and Binnewies, {Tim T.} and Hallin, {Peter F.} and Guilin Wang and Joel Malek and Andrea Rogosin and Stanker, {Larry H.} and Mandrell, {Robert E.}",
year = "2007",
month = "12",
day = "26",
doi = "10.1371/journal.pone.0001358",
language = "English",
volume = "2",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "12",

}

TY - JOUR

T1 - The complete genome sequence and analysis of the epsilonproteobacterium Arcobacter butzleri

AU - Miller, William G.

AU - Parker, Craig T.

AU - Rubenfield, Marc

AU - Mendz, George L.

AU - Wösten, Marc M.S.M.

AU - Ussery, David W.

AU - Stolz, John F.

AU - Binnewies, Tim T.

AU - Hallin, Peter F.

AU - Wang, Guilin

AU - Malek, Joel

AU - Rogosin, Andrea

AU - Stanker, Larry H.

AU - Mandrell, Robert E.

PY - 2007/12/26

Y1 - 2007/12/26

N2 - Background. Arcobacter butzleri is a member of the epsilon subdivision of the Proteobacteria and a close taxonomic relative of established pathogens, such as Campylobacter jejuni and Helicobacter pylori. Here we present the complete genome sequence of the human clinical isolate, A. butzleri strain RM4018. Methodology/Principal Findings. Arcobacter butzleri is a member of the Campylobacteraceae, but the majority of its proteome is most similar to those of Sulfuromonas denitrificans and Wolinella succinogenes, both members of the Helicobacteraceae, and those of the deep-sea vent Epsilonproteobacteria Sulfurovum and Nitratiruptor. In addition, many of the genes and pathways described here, e.g. those involved in signal transduction and sulfur metabolism, have been identified previously within the epsilon subdivision only in S. denitrificans, W. succinogenes, Sulfurovum, and/or Nitratiruptor, or are unique to the subdivision. In addition, the analyses indicated also that a substantial proportion of the A. butzleri genome is devoted to growth and survival under diverse environmental conditions, with a large number of respiration-associated proteins, signal transduction and chemotaxis proteins and proteins involved in DNA repair and adaptation. To investigate the genomic diversity of A. butzleri strains, we constructed an A. butzleri DNA microarray comprising 2238 genes from strain RM4018. Comparative genomic indexing analysis of 12 additional A. butzleri strains identified both the core genes of A. butzleri and intraspecies hypervariable regions, where <70% of the genes were present in at least two strains. Conclusion/Significance. The presence of pathways and loci associated often with non-host-associated organisms, as well as genes associated with virulence, suggests that A. butzleri is a free-living, water-borne organism that might be classified rightfully as an emerging pathogen. The genome sequence and analyses presented in this study are an important first step in understanding the physiology and genetics of this organism, which constitutes a bridge between the environment and mammalian hosts.

AB - Background. Arcobacter butzleri is a member of the epsilon subdivision of the Proteobacteria and a close taxonomic relative of established pathogens, such as Campylobacter jejuni and Helicobacter pylori. Here we present the complete genome sequence of the human clinical isolate, A. butzleri strain RM4018. Methodology/Principal Findings. Arcobacter butzleri is a member of the Campylobacteraceae, but the majority of its proteome is most similar to those of Sulfuromonas denitrificans and Wolinella succinogenes, both members of the Helicobacteraceae, and those of the deep-sea vent Epsilonproteobacteria Sulfurovum and Nitratiruptor. In addition, many of the genes and pathways described here, e.g. those involved in signal transduction and sulfur metabolism, have been identified previously within the epsilon subdivision only in S. denitrificans, W. succinogenes, Sulfurovum, and/or Nitratiruptor, or are unique to the subdivision. In addition, the analyses indicated also that a substantial proportion of the A. butzleri genome is devoted to growth and survival under diverse environmental conditions, with a large number of respiration-associated proteins, signal transduction and chemotaxis proteins and proteins involved in DNA repair and adaptation. To investigate the genomic diversity of A. butzleri strains, we constructed an A. butzleri DNA microarray comprising 2238 genes from strain RM4018. Comparative genomic indexing analysis of 12 additional A. butzleri strains identified both the core genes of A. butzleri and intraspecies hypervariable regions, where <70% of the genes were present in at least two strains. Conclusion/Significance. The presence of pathways and loci associated often with non-host-associated organisms, as well as genes associated with virulence, suggests that A. butzleri is a free-living, water-borne organism that might be classified rightfully as an emerging pathogen. The genome sequence and analyses presented in this study are an important first step in understanding the physiology and genetics of this organism, which constitutes a bridge between the environment and mammalian hosts.

UR - http://www.scopus.com/inward/record.url?scp=43349108455&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=43349108455&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0001358

DO - 10.1371/journal.pone.0001358

M3 - Article

VL - 2

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 12

M1 - e1358

ER -