The CEPH YAC library.

J. Dausset, P. Ougen, H. Abderrahim, A. Billault, J. L. Sambucy, D. Cohen, D. Le Paslier

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Because of their large size, YACs provide is a powerful tool for physical mapping studies of complex genomes. As it will be advantageous to have genomic libraries of clones with large inserts for analyzing megabase sized regions of the human genome, we have investigated a number of parameters in order to increase the insert size of the YACs. We constructed a genomic library currently containing more than 85,000 YAC clones. Mean sizes of YACs produced at several stages of construction of the library range from 430 kb to 1,200 kb, representing 13 haploid equivalents of the human genome. This library was organized in order to allow rapid screen of YACs for large scale physical mapping of the human genome.

Original languageEnglish
Pages (from-to)13-20
Number of pages8
JournalBehring Institute Mitteilungen
Issue number91
Publication statusPublished - 1 Apr 1992
Externally publishedYes

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Human Genome
Libraries
Genomic Library
Genes
Clone Cells
Haploidy
Genome

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Dausset, J., Ougen, P., Abderrahim, H., Billault, A., Sambucy, J. L., Cohen, D., & Le Paslier, D. (1992). The CEPH YAC library. Behring Institute Mitteilungen, (91), 13-20.

The CEPH YAC library. / Dausset, J.; Ougen, P.; Abderrahim, H.; Billault, A.; Sambucy, J. L.; Cohen, D.; Le Paslier, D.

In: Behring Institute Mitteilungen, No. 91, 01.04.1992, p. 13-20.

Research output: Contribution to journalArticle

Dausset, J, Ougen, P, Abderrahim, H, Billault, A, Sambucy, JL, Cohen, D & Le Paslier, D 1992, 'The CEPH YAC library.', Behring Institute Mitteilungen, no. 91, pp. 13-20.
Dausset J, Ougen P, Abderrahim H, Billault A, Sambucy JL, Cohen D et al. The CEPH YAC library. Behring Institute Mitteilungen. 1992 Apr 1;(91):13-20.
Dausset, J. ; Ougen, P. ; Abderrahim, H. ; Billault, A. ; Sambucy, J. L. ; Cohen, D. ; Le Paslier, D. / The CEPH YAC library. In: Behring Institute Mitteilungen. 1992 ; No. 91. pp. 13-20.
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