The CDX1-microRNA-215 axis regulates colorectal cancer stem cell differentiation

Matthew F. Jones, Toshifumi Hara, Princy Francis, Xiao Ling Li, Sven Bilke, Yuelin Zhu, Marbin Pineda, Murugan Subramanian, Walter F. Bodmer, Ashish Lal

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

The transcription factor caudal-type homeobox 1 (CDX1) is a key regulator of differentiation in the normal colon and in colorectal cancer (CRC). CDX1 activates the expression of enterocyte genes, but it is not clear how the concomitant silencing of stem cell genes is achieved. MicroRNAs (miRNAs) are important mediators of gene repression and have been implicated in tumor suppression and carcinogenesis, but the roles of miRNAs in differentiation, particularly in CRC, remain poorly understood. Here, we identified microRNA-215 (miR-215) as a direct transcriptional target of CDX1 by using highthroughput small RNA sequencing to profile miRNA expression in two pairs of CRC cell lines: CDX1-low HCT116 and HCT116 with stable CDX1 overexpression, and CDX1-high LS174T and LS174T with stable CDX1 knockdown. Validation of candidate miRNAs identified by RNA-seq in a larger cell-line panel revealed miR-215 to be most significantly correlated with CDX1 expression. Quantitative ChIP-PCR and promoter luciferase assays confirmed that CDX1 directly activates miR-215 transcription. miR-215 expression is depleted in FACS-enriched cancer stem cells compared with unsorted samples. Overexpression of miR-215 in poorly differentiated cell lines causes a decrease in clonogenicity, whereas miR-215 knockdown increases clonogenicity and impairs differentiation in CDX1-high cell lines. We identified the genome-wide targets of miR-215 and found that miR-215 mediates the repression of cell cycle and stemness genes downstream of CDX1. In particular, the miR-215 target gene BMI1 has been shown to promote stemness and self-renewal and to vary inversely with CDX1. Our work situates miR-215 as a link between CDX1 expression and BMI1 repression that governs differentiation in CRC.

Original languageEnglish
Pages (from-to)E1550-E1558
JournalProceedings of the National Academy of Sciences of the United States of America
Volume112
Issue number13
DOIs
Publication statusPublished - 31 Mar 2015
Externally publishedYes

Fingerprint

Neoplastic Stem Cells
Homeobox Genes
MicroRNAs
Cell Differentiation
Colorectal Neoplasms
Cell Line
Genes
RNA Sequence Analysis
cdc Genes
Enterocytes
Luciferases
Colon
Carcinogenesis

Keywords

  • Cancer stem cells
  • CDX1
  • Colorectal cancer
  • MiR-215
  • MiRNA

ASJC Scopus subject areas

  • General

Cite this

The CDX1-microRNA-215 axis regulates colorectal cancer stem cell differentiation. / Jones, Matthew F.; Hara, Toshifumi; Francis, Princy; Li, Xiao Ling; Bilke, Sven; Zhu, Yuelin; Pineda, Marbin; Subramanian, Murugan; Bodmer, Walter F.; Lal, Ashish.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, No. 13, 31.03.2015, p. E1550-E1558.

Research output: Contribution to journalArticle

Jones, Matthew F. ; Hara, Toshifumi ; Francis, Princy ; Li, Xiao Ling ; Bilke, Sven ; Zhu, Yuelin ; Pineda, Marbin ; Subramanian, Murugan ; Bodmer, Walter F. ; Lal, Ashish. / The CDX1-microRNA-215 axis regulates colorectal cancer stem cell differentiation. In: Proceedings of the National Academy of Sciences of the United States of America. 2015 ; Vol. 112, No. 13. pp. E1550-E1558.
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AU - Zhu, Yuelin

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