The Ca2+-activated Cl- channel Ano1 controls microvilli length and membrane surface area in the oocyte

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7 Citations (Scopus)

Abstract

Ca2+-activated Cl- channels (CaCCs) play important physiological functions in epithelia and other tissues. In frog oocytes the CaCC Ano1 regulates resting membrane potential and the block to polyspermy. Here, we show that Ano1 expression increases the oocyte surface, revealing a novel function for Ano1 in regulating cell morphology. Confocal imaging shows that Ano1 increases microvilli length, which requires ERM-protein-dependent linkage to the cytoskeleton. A dominant-negative form of the ERM protein moesin precludes the Ano1-dependent increase in membrane area. Furthermore, both full-length and the truncated dominant-negative forms of moesin co-localize with Ano1 to the microvilli, and the two proteins co-immunoprecipitate. The Ano1-moesin interaction limits Ano1 lateral membrane mobility and contributes to microvilli scaffolding, therefore stabilizing larger membrane structures. Collectively, these results reveal a newly identified role for Ano1 in shaping the plasma membrane during oogenesis, with broad implications for the regulation of microvilli in epithelia.

Original languageEnglish
Pages (from-to)2548-2558
Number of pages11
JournalJournal of Cell Science
Volume129
Issue number13
DOIs
Publication statusPublished - 2016

Fingerprint

Microvilli
Oocytes
Membranes
Epithelium
Oogenesis
Proteins
Cytoskeleton
Anura
Membrane Potentials
Cell Membrane
moesin

Keywords

  • Ano1
  • ERM
  • Microvilli
  • Moesin
  • Xenopus oocyte

ASJC Scopus subject areas

  • Cell Biology

Cite this

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title = "The Ca2+-activated Cl- channel Ano1 controls microvilli length and membrane surface area in the oocyte",
abstract = "Ca2+-activated Cl- channels (CaCCs) play important physiological functions in epithelia and other tissues. In frog oocytes the CaCC Ano1 regulates resting membrane potential and the block to polyspermy. Here, we show that Ano1 expression increases the oocyte surface, revealing a novel function for Ano1 in regulating cell morphology. Confocal imaging shows that Ano1 increases microvilli length, which requires ERM-protein-dependent linkage to the cytoskeleton. A dominant-negative form of the ERM protein moesin precludes the Ano1-dependent increase in membrane area. Furthermore, both full-length and the truncated dominant-negative forms of moesin co-localize with Ano1 to the microvilli, and the two proteins co-immunoprecipitate. The Ano1-moesin interaction limits Ano1 lateral membrane mobility and contributes to microvilli scaffolding, therefore stabilizing larger membrane structures. Collectively, these results reveal a newly identified role for Ano1 in shaping the plasma membrane during oogenesis, with broad implications for the regulation of microvilli in epithelia.",
keywords = "Ano1, ERM, Microvilli, Moesin, Xenopus oocyte",
author = "Courjaret, {Raphael Jean} and Rawad Hodeify and Hubrack, {Satanay Zuhair} and Awab Ibrahim and Maya Dib and Sahar Da'as and Khaled Machaca",
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AU - Courjaret, Raphael Jean

AU - Hodeify, Rawad

AU - Hubrack, Satanay Zuhair

AU - Ibrahim, Awab

AU - Dib, Maya

AU - Da'as, Sahar

AU - Machaca, Khaled

PY - 2016

Y1 - 2016

N2 - Ca2+-activated Cl- channels (CaCCs) play important physiological functions in epithelia and other tissues. In frog oocytes the CaCC Ano1 regulates resting membrane potential and the block to polyspermy. Here, we show that Ano1 expression increases the oocyte surface, revealing a novel function for Ano1 in regulating cell morphology. Confocal imaging shows that Ano1 increases microvilli length, which requires ERM-protein-dependent linkage to the cytoskeleton. A dominant-negative form of the ERM protein moesin precludes the Ano1-dependent increase in membrane area. Furthermore, both full-length and the truncated dominant-negative forms of moesin co-localize with Ano1 to the microvilli, and the two proteins co-immunoprecipitate. The Ano1-moesin interaction limits Ano1 lateral membrane mobility and contributes to microvilli scaffolding, therefore stabilizing larger membrane structures. Collectively, these results reveal a newly identified role for Ano1 in shaping the plasma membrane during oogenesis, with broad implications for the regulation of microvilli in epithelia.

AB - Ca2+-activated Cl- channels (CaCCs) play important physiological functions in epithelia and other tissues. In frog oocytes the CaCC Ano1 regulates resting membrane potential and the block to polyspermy. Here, we show that Ano1 expression increases the oocyte surface, revealing a novel function for Ano1 in regulating cell morphology. Confocal imaging shows that Ano1 increases microvilli length, which requires ERM-protein-dependent linkage to the cytoskeleton. A dominant-negative form of the ERM protein moesin precludes the Ano1-dependent increase in membrane area. Furthermore, both full-length and the truncated dominant-negative forms of moesin co-localize with Ano1 to the microvilli, and the two proteins co-immunoprecipitate. The Ano1-moesin interaction limits Ano1 lateral membrane mobility and contributes to microvilli scaffolding, therefore stabilizing larger membrane structures. Collectively, these results reveal a newly identified role for Ano1 in shaping the plasma membrane during oogenesis, with broad implications for the regulation of microvilli in epithelia.

KW - Ano1

KW - ERM

KW - Microvilli

KW - Moesin

KW - Xenopus oocyte

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