Tethered polymer layers: Phase transitions and reduction of protein adsorption

I. Szleifer, Marcelo Carignano

Research output: Contribution to journalArticle

117 Citations (Scopus)

Abstract

Feature Article: The structural and thermodynamic properties of tethered polymer layers formed by spreading diblock copolymers at a solid surface or at a fluid-fluid interface are studied using a molecular mean-field theory. The role of the anchoring block in determining the properties of the tethered polymer layer is studied in detail. It is found that both the anchoring and the tethered blocks are very important in determining the phase behavior of the polymer layer. The structures of the coexisting phases, the phase boundaries and the stability of the layer are found to depend on the ratio of molecular weight between the two blocks, the polymer-interface (surface) interactions and the strength of the interactions between the two blocks. The different phase transitions found are related to experimental observations. The properties of the polymer layers at coexistence reflect the block that is the dominant driving force for phase separation. The ability of the tethered polymer layers, under different conditions, to control protein adsorption to surfaces is also studied. It is found that the most important factors determining the ability of a polymer layer to reduce the equilibrium amount of proteins adsorbed to a surface are the surface coverage of polymer and the surface-polymer interactions. The polymer chain length plays only a secondary role. For the kinetic control, however, it is found that the potential of mean-force, and thus the early stages of adsorption, depends strongly on polymer molecular weight. Further, it is found that the molecular factors determining the ability of the tethered polymer layer to reduce the equilibrium amount of protein adsorption are different than those that control the kinetic behavior. Comparisons with experimental observations are presented. The predictions of the theory are in very good agreement with the measured adsorption isotherms. Guidelines for building optimal surface protection for protein adsorption, both kinetic and thermodynamic, are discussed.

Original languageEnglish
Pages (from-to)423-448
Number of pages26
JournalMacromolecular Rapid Communications
Volume21
Issue number8
Publication statusPublished - 31 May 2000
Externally publishedYes

Fingerprint

Polymers
Phase transitions
Proteins
Adsorption
Kinetics
Molecular weight
Mean field theory
Fluids
Phase boundaries
Phase behavior
Chain length
Adsorption isotherms
Phase separation
Block copolymers
Structural properties
Thermodynamic properties
Thermodynamics

ASJC Scopus subject areas

  • Materials Chemistry
  • Polymers and Plastics

Cite this

Tethered polymer layers : Phase transitions and reduction of protein adsorption. / Szleifer, I.; Carignano, Marcelo.

In: Macromolecular Rapid Communications, Vol. 21, No. 8, 31.05.2000, p. 423-448.

Research output: Contribution to journalArticle

@article{c0ad647ae9e64580afa4d69f07fde1d1,
title = "Tethered polymer layers: Phase transitions and reduction of protein adsorption",
abstract = "Feature Article: The structural and thermodynamic properties of tethered polymer layers formed by spreading diblock copolymers at a solid surface or at a fluid-fluid interface are studied using a molecular mean-field theory. The role of the anchoring block in determining the properties of the tethered polymer layer is studied in detail. It is found that both the anchoring and the tethered blocks are very important in determining the phase behavior of the polymer layer. The structures of the coexisting phases, the phase boundaries and the stability of the layer are found to depend on the ratio of molecular weight between the two blocks, the polymer-interface (surface) interactions and the strength of the interactions between the two blocks. The different phase transitions found are related to experimental observations. The properties of the polymer layers at coexistence reflect the block that is the dominant driving force for phase separation. The ability of the tethered polymer layers, under different conditions, to control protein adsorption to surfaces is also studied. It is found that the most important factors determining the ability of a polymer layer to reduce the equilibrium amount of proteins adsorbed to a surface are the surface coverage of polymer and the surface-polymer interactions. The polymer chain length plays only a secondary role. For the kinetic control, however, it is found that the potential of mean-force, and thus the early stages of adsorption, depends strongly on polymer molecular weight. Further, it is found that the molecular factors determining the ability of the tethered polymer layer to reduce the equilibrium amount of protein adsorption are different than those that control the kinetic behavior. Comparisons with experimental observations are presented. The predictions of the theory are in very good agreement with the measured adsorption isotherms. Guidelines for building optimal surface protection for protein adsorption, both kinetic and thermodynamic, are discussed.",
author = "I. Szleifer and Marcelo Carignano",
year = "2000",
month = "5",
day = "31",
language = "English",
volume = "21",
pages = "423--448",
journal = "Macromolecular Rapid Communications",
issn = "1022-1336",
publisher = "Wiley-VCH Verlag",
number = "8",

}

TY - JOUR

T1 - Tethered polymer layers

T2 - Phase transitions and reduction of protein adsorption

AU - Szleifer, I.

AU - Carignano, Marcelo

PY - 2000/5/31

Y1 - 2000/5/31

N2 - Feature Article: The structural and thermodynamic properties of tethered polymer layers formed by spreading diblock copolymers at a solid surface or at a fluid-fluid interface are studied using a molecular mean-field theory. The role of the anchoring block in determining the properties of the tethered polymer layer is studied in detail. It is found that both the anchoring and the tethered blocks are very important in determining the phase behavior of the polymer layer. The structures of the coexisting phases, the phase boundaries and the stability of the layer are found to depend on the ratio of molecular weight between the two blocks, the polymer-interface (surface) interactions and the strength of the interactions between the two blocks. The different phase transitions found are related to experimental observations. The properties of the polymer layers at coexistence reflect the block that is the dominant driving force for phase separation. The ability of the tethered polymer layers, under different conditions, to control protein adsorption to surfaces is also studied. It is found that the most important factors determining the ability of a polymer layer to reduce the equilibrium amount of proteins adsorbed to a surface are the surface coverage of polymer and the surface-polymer interactions. The polymer chain length plays only a secondary role. For the kinetic control, however, it is found that the potential of mean-force, and thus the early stages of adsorption, depends strongly on polymer molecular weight. Further, it is found that the molecular factors determining the ability of the tethered polymer layer to reduce the equilibrium amount of protein adsorption are different than those that control the kinetic behavior. Comparisons with experimental observations are presented. The predictions of the theory are in very good agreement with the measured adsorption isotherms. Guidelines for building optimal surface protection for protein adsorption, both kinetic and thermodynamic, are discussed.

AB - Feature Article: The structural and thermodynamic properties of tethered polymer layers formed by spreading diblock copolymers at a solid surface or at a fluid-fluid interface are studied using a molecular mean-field theory. The role of the anchoring block in determining the properties of the tethered polymer layer is studied in detail. It is found that both the anchoring and the tethered blocks are very important in determining the phase behavior of the polymer layer. The structures of the coexisting phases, the phase boundaries and the stability of the layer are found to depend on the ratio of molecular weight between the two blocks, the polymer-interface (surface) interactions and the strength of the interactions between the two blocks. The different phase transitions found are related to experimental observations. The properties of the polymer layers at coexistence reflect the block that is the dominant driving force for phase separation. The ability of the tethered polymer layers, under different conditions, to control protein adsorption to surfaces is also studied. It is found that the most important factors determining the ability of a polymer layer to reduce the equilibrium amount of proteins adsorbed to a surface are the surface coverage of polymer and the surface-polymer interactions. The polymer chain length plays only a secondary role. For the kinetic control, however, it is found that the potential of mean-force, and thus the early stages of adsorption, depends strongly on polymer molecular weight. Further, it is found that the molecular factors determining the ability of the tethered polymer layer to reduce the equilibrium amount of protein adsorption are different than those that control the kinetic behavior. Comparisons with experimental observations are presented. The predictions of the theory are in very good agreement with the measured adsorption isotherms. Guidelines for building optimal surface protection for protein adsorption, both kinetic and thermodynamic, are discussed.

UR - http://www.scopus.com/inward/record.url?scp=23044519690&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=23044519690&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:23044519690

VL - 21

SP - 423

EP - 448

JO - Macromolecular Rapid Communications

JF - Macromolecular Rapid Communications

SN - 1022-1336

IS - 8

ER -