Targeted label-free quantitative analysis of secretory proteins from adipocytes in response to oxidative stress

Sunkyu Choi, Jaeyoon Kim, Kyungmoo Yea, Pann Ghill Suh, Jeongkwon Kim, Sung Ho Ryu

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Adipocytes are well known to release regulation factors associated with metabolic disorders. In particular, increased oxidative stress in adipocytes contributes to dysregulation of adipokine production. In this study, we applied relative quantitative proteomic analysis based on label-free multiple reaction monitoring (MRM) to discover biological changes of adipokines under oxidative stress. Among a total of 194 identified proteins, 8 proteins were selected and quantified between control and hydrogen peroxide (H2O2)-treated groups by label-free MRM quantification. The secretion levels of matrix metalloproteinase-2 (MMP-2), stromal cell-derived factor-1 (SDF-1, CXCL12), resistin, and complement factor D (CFD, adipsin) decreased, whereas the secretion levels of tissue inhibitor of metalloproteinase-2 (TIMP-2) and aldolase A increased. Here we suggest that our study with label-free quantitative analysis will contribute to the efficient quantitative analysis of targeted proteins in complex mixtures and specifically to a better understanding of changes of adipokines under oxidative stress.

Original languageEnglish
Pages (from-to)196-202
Number of pages7
JournalAnalytical Biochemistry
Volume401
Issue number2
DOIs
Publication statusPublished - 1 Jun 2010
Externally publishedYes

Fingerprint

Adipokines
Oxidative stress
Adipocytes
Complement Factor D
Labels
Oxidative Stress
Chemical analysis
Resistin
Chemokine CXCL12
Tissue Inhibitor of Metalloproteinase-2
Fructose-Bisphosphate Aldolase
Proteins
Monitoring
Matrix Metalloproteinase 2
Complex Mixtures
Proteomics
Hydrogen Peroxide
Computational fluid dynamics

Keywords

  • Adipocytes
  • Adipokines
  • Label-free quantification
  • Multiple reaction monitoring
  • Oxidative stress
  • Triple quadrupole mass spectrometry

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology

Cite this

Targeted label-free quantitative analysis of secretory proteins from adipocytes in response to oxidative stress. / Choi, Sunkyu; Kim, Jaeyoon; Yea, Kyungmoo; Suh, Pann Ghill; Kim, Jeongkwon; Ryu, Sung Ho.

In: Analytical Biochemistry, Vol. 401, No. 2, 01.06.2010, p. 196-202.

Research output: Contribution to journalArticle

Choi, Sunkyu ; Kim, Jaeyoon ; Yea, Kyungmoo ; Suh, Pann Ghill ; Kim, Jeongkwon ; Ryu, Sung Ho. / Targeted label-free quantitative analysis of secretory proteins from adipocytes in response to oxidative stress. In: Analytical Biochemistry. 2010 ; Vol. 401, No. 2. pp. 196-202.
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