Targeted killing of yeast expressing a HIV-1 peptide by antibody-conjugated glucose oxidase and horseradish peroxidase

Lotfi Chouchane, T. Bringman, S. Barbier, F. Traincard, A. Donny Strosberg

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The epitope recognized by monoclonal antibody directed against the HIV-1 recombinant gp160 protein was precisely delineated by using a number of peptides comprising amino acid positions 302-330 of the protein. Two different enzymes, glucose oxidase and horseradish peroxidase, were then coupled to distinct antibody molecules and the efficacy of the immunoenzymes in killing yeast cells which express the recognized peptide was evaluated by flow cytometry analysis. The antibody-glucose oxidase conjugate alone was cytotoxic only at large doses (over 35 μg/ml) while in the presence of the antibody-horseradish peroxidase conjugate, killing was observed at nine times lower concentrations (4 μg/ml). The procedure described here may provide a new immunotherapy tool for microbial infection.

Original languageEnglish
Pages (from-to)359-365
Number of pages7
JournalImmunology Letters
Volume25
Issue number4
DOIs
Publication statusPublished - 1990
Externally publishedYes

Fingerprint

Glucose Oxidase
Horseradish Peroxidase
HIV-1
Yeasts
Peptides
Antibodies
Recombinant Proteins
Immunotherapy
Epitopes
Flow Cytometry
Monoclonal Antibodies
Amino Acids
Enzymes
Infection
glucose peroxidase
Proteins

Keywords

  • Antibody-conjugated enzyme
  • HIV-1
  • Targeted killing

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Targeted killing of yeast expressing a HIV-1 peptide by antibody-conjugated glucose oxidase and horseradish peroxidase. / Chouchane, Lotfi; Bringman, T.; Barbier, S.; Traincard, F.; Strosberg, A. Donny.

In: Immunology Letters, Vol. 25, No. 4, 1990, p. 359-365.

Research output: Contribution to journalArticle

Chouchane, Lotfi ; Bringman, T. ; Barbier, S. ; Traincard, F. ; Strosberg, A. Donny. / Targeted killing of yeast expressing a HIV-1 peptide by antibody-conjugated glucose oxidase and horseradish peroxidase. In: Immunology Letters. 1990 ; Vol. 25, No. 4. pp. 359-365.
@article{9b17501c6e03407a823100a298b11ca8,
title = "Targeted killing of yeast expressing a HIV-1 peptide by antibody-conjugated glucose oxidase and horseradish peroxidase",
abstract = "The epitope recognized by monoclonal antibody directed against the HIV-1 recombinant gp160 protein was precisely delineated by using a number of peptides comprising amino acid positions 302-330 of the protein. Two different enzymes, glucose oxidase and horseradish peroxidase, were then coupled to distinct antibody molecules and the efficacy of the immunoenzymes in killing yeast cells which express the recognized peptide was evaluated by flow cytometry analysis. The antibody-glucose oxidase conjugate alone was cytotoxic only at large doses (over 35 μg/ml) while in the presence of the antibody-horseradish peroxidase conjugate, killing was observed at nine times lower concentrations (4 μg/ml). The procedure described here may provide a new immunotherapy tool for microbial infection.",
keywords = "Antibody-conjugated enzyme, HIV-1, Targeted killing",
author = "Lotfi Chouchane and T. Bringman and S. Barbier and F. Traincard and Strosberg, {A. Donny}",
year = "1990",
doi = "10.1016/0165-2478(90)90208-8",
language = "English",
volume = "25",
pages = "359--365",
journal = "Immunology Letters",
issn = "0165-2478",
publisher = "Elsevier",
number = "4",

}

TY - JOUR

T1 - Targeted killing of yeast expressing a HIV-1 peptide by antibody-conjugated glucose oxidase and horseradish peroxidase

AU - Chouchane, Lotfi

AU - Bringman, T.

AU - Barbier, S.

AU - Traincard, F.

AU - Strosberg, A. Donny

PY - 1990

Y1 - 1990

N2 - The epitope recognized by monoclonal antibody directed against the HIV-1 recombinant gp160 protein was precisely delineated by using a number of peptides comprising amino acid positions 302-330 of the protein. Two different enzymes, glucose oxidase and horseradish peroxidase, were then coupled to distinct antibody molecules and the efficacy of the immunoenzymes in killing yeast cells which express the recognized peptide was evaluated by flow cytometry analysis. The antibody-glucose oxidase conjugate alone was cytotoxic only at large doses (over 35 μg/ml) while in the presence of the antibody-horseradish peroxidase conjugate, killing was observed at nine times lower concentrations (4 μg/ml). The procedure described here may provide a new immunotherapy tool for microbial infection.

AB - The epitope recognized by monoclonal antibody directed against the HIV-1 recombinant gp160 protein was precisely delineated by using a number of peptides comprising amino acid positions 302-330 of the protein. Two different enzymes, glucose oxidase and horseradish peroxidase, were then coupled to distinct antibody molecules and the efficacy of the immunoenzymes in killing yeast cells which express the recognized peptide was evaluated by flow cytometry analysis. The antibody-glucose oxidase conjugate alone was cytotoxic only at large doses (over 35 μg/ml) while in the presence of the antibody-horseradish peroxidase conjugate, killing was observed at nine times lower concentrations (4 μg/ml). The procedure described here may provide a new immunotherapy tool for microbial infection.

KW - Antibody-conjugated enzyme

KW - HIV-1

KW - Targeted killing

UR - http://www.scopus.com/inward/record.url?scp=0025120568&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025120568&partnerID=8YFLogxK

U2 - 10.1016/0165-2478(90)90208-8

DO - 10.1016/0165-2478(90)90208-8

M3 - Article

C2 - 1701163

AN - SCOPUS:0025120568

VL - 25

SP - 359

EP - 365

JO - Immunology Letters

JF - Immunology Letters

SN - 0165-2478

IS - 4

ER -