Tailoring DNA Structure to increase target hybridization kinetics on surfaces

Andrew E. Prigodich, One-Sun Lee, Weston L. Daniel, Dwight S. Seferos, George C. Schatz, Chad A. Mirkin

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59 Citations (Scopus)

Abstract

We report a method for increasing the rate of target hybridization on DNA-functionalized surfaces using a short internal complement DNA (sicDNA) strand. The sicDNA causes up to a 5-fold increase in association rate by inducing a conformational change that extends the DNA away from the surface, making it more available to bind target nucleic acids. The sicDNA-induced kinetic enhancement is a general phenomenon that occurred with all sequences and surfaces investigated. Additionally, the process is selective and can be used in multicomponent systems to controllably and orthogonally "turn on" specific sequences by the addition of the appropriate sicDNA. Finally, we show that sicDNA is compatible with systems used in gene regulation, intracellular detection, and microarrays, suggesting several potential therapeutic, diagnostic, and bioinformatic applications.

Original languageEnglish
Pages (from-to)10638-10641
Number of pages4
JournalJournal of the American Chemical Society
Volume132
Issue number31
DOIs
Publication statusPublished - 11 Aug 2010
Externally publishedYes

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ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Prigodich, A. E., Lee, O-S., Daniel, W. L., Seferos, D. S., Schatz, G. C., & Mirkin, C. A. (2010). Tailoring DNA Structure to increase target hybridization kinetics on surfaces. Journal of the American Chemical Society, 132(31), 10638-10641. https://doi.org/10.1021/ja104859j