T Helper 17-Associated Cytokines Are Produced during Antigen-Specific Inflammation in the Mammary Gland

Pascal Rainard, Patricia Cunha, Salim Bougarn, Angélina Fromageau, Christelle Rossignol, Florence B. Gilbert, Patricia Berthon

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Infectious mastitis cuts down milk production profitability and is a major animal welfare problem. Bacteria-induced inflammation in the mammary gland (MG) is driven by innate immunity, but adaptive immunity can modulate the innate response. Several studies have shown that it is possible to elicit inflammation in the MG by sensitization to an antigen subsequently infused into the lumen of the gland. The objective of our study was to characterize the inflammation triggered in the MG of cows sensitized to ovalbumin, by identifying the cytokines and chemokines likely to play a part in the reaction. Among immunized cows, responders mobilized locally high numbers of leukocytes. An overexpression of the genes encoding IL-17a, IL-17F, IL-21, IL-22 and INF-γ was found in milk cell RNA extracts in the early phase of the inflammatory response. At the protein level, IL-17A was detected in milk as soon as the first sampling time (8 h post-challenge), and both IL-17A and IFN-γ concentrations peaked at 12 to 24 h post-challenge. In mammary tissue from challenged quarters, overexpression of the genes encoding IL-17A, IL-17F, IL-21, IL-22, IL-26 and IFN-γ was observed. Neutrophil-attracting chemokines (CXCL3 and CXCL8) were found in milk, and overexpressed transcripts of chemokines attracting lymphocytes and other mononuclear leukocytes (CXCL10, CCL2, CCL5, CCL20) were detected in mammary tissue. Expression of IL-17A, as revealed by immunohistochemistry, was located in epithelial cells, in leukocytes in the connective tissue and in association with the epithelium, and in migrated alveolar leukocytes of challenged quarters. Altogether, these results show that antigen-specific inflammation in the MG was characterized by the production of IL-17 and IFN-γ. The orientation of the inflammatory response induced by the antigen-specific response has the potential to strongly impact the outcome of bacterial infections of the MG.

Original languageEnglish
Article numbere63471
JournalPLoS One
Volume8
Issue number5
DOIs
Publication statusPublished - 16 May 2013
Externally publishedYes

Fingerprint

Interleukin-17
Human Mammary Glands
mammary glands
cytokines
inflammation
Cytokines
Inflammation
antigens
Antigens
Milk
gene overexpression
Chemokines
chemokines
milk
Gene encoding
breasts
leukocytes
Tissue
cows
Breast

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Rainard, P., Cunha, P., Bougarn, S., Fromageau, A., Rossignol, C., Gilbert, F. B., & Berthon, P. (2013). T Helper 17-Associated Cytokines Are Produced during Antigen-Specific Inflammation in the Mammary Gland. PLoS One, 8(5), [e63471]. https://doi.org/10.1371/journal.pone.0063471

T Helper 17-Associated Cytokines Are Produced during Antigen-Specific Inflammation in the Mammary Gland. / Rainard, Pascal; Cunha, Patricia; Bougarn, Salim; Fromageau, Angélina; Rossignol, Christelle; Gilbert, Florence B.; Berthon, Patricia.

In: PLoS One, Vol. 8, No. 5, e63471, 16.05.2013.

Research output: Contribution to journalArticle

Rainard, Pascal ; Cunha, Patricia ; Bougarn, Salim ; Fromageau, Angélina ; Rossignol, Christelle ; Gilbert, Florence B. ; Berthon, Patricia. / T Helper 17-Associated Cytokines Are Produced during Antigen-Specific Inflammation in the Mammary Gland. In: PLoS One. 2013 ; Vol. 8, No. 5.
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