T cell immunity to the alkyl hydroperoxide reductase of Burkholderia pseudomallei

A correlate of disease outcome in acute melioidosis

Catherine Reynolds, Amélie Goudet, Kemajittra Jenjaroen, Manutsanun Sumonwiriya, Darawan Rinchai, Julie Musson, Saskia Overbeek, Julia Makinde, Kathryn Quigley, Jiten Manji, Natasha Spink, Pagnarith Yos, Vanaporn Wuthiekanun, Gregory Bancroft, John Robinson, Ganjana Lertmemongkolchai, Susanna Dunachie, Bernard Maillere, Matthew Holden, Daniel Altmann & 1 others Rosemary Boyton

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

There is an urgent need for a better understanding of adaptive immunity to Burkholderia pseudomallei, the causative agent of melioidosis that is frequently associated with sepsis or death in patients in Southeast Asia and Northern Australia. The imperative to identify vaccine targets is driven both by the public health agenda in these regions and biological threat concerns. In several intracellular bacterial pathogens, alkyl hydroperoxidase reductases are upregulated as part of the response to host oxidative stress, and they can stimulate strong adaptive immunity. We show that alkyl hydroperoxidase reductase (AhpC) of B. pseudomallei is strongly immunogenic for T cells of 'humanized' HLA transgenic mice and seropositive human donors. Some T cell epitopes, such as p6, are able to bind diverse HLA class II heterodimers and stimulate strong T cell immunity in mice and humans. Importantly, patients with acute melioidosis who survive infection show stronger T cell responses to AhpC relative to those who do not. Although the sequence of AhpC is virtually invariant among global B. pseudomallei clinical isolates, a Cambodian isolate varies only in C-terminal truncation of the p6 T cell epitope, raising the possibility of selection by host immunity. This variant peptide is virtually unable to stimulate T cell immunity. For an infection in which there has been debate about centrality of T cell immunity in defense, these observations support a role for T cell immunity to AhpC in disease protection.

Original languageEnglish
Pages (from-to)4814-4824
Number of pages11
JournalJournal of Immunology
Volume194
Issue number10
DOIs
Publication statusPublished - 1 Jan 2015
Externally publishedYes

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Melioidosis
Burkholderia pseudomallei
Peroxiredoxins
Immunity
Oxidoreductases
T-Lymphocytes
T-Lymphocyte Epitopes
Adaptive Immunity
Southeastern Asia
Infection
Transgenic Mice
Sepsis
Oxidative Stress
Vaccines
Public Health
Tissue Donors
Peptides

ASJC Scopus subject areas

  • Immunology

Cite this

T cell immunity to the alkyl hydroperoxide reductase of Burkholderia pseudomallei : A correlate of disease outcome in acute melioidosis. / Reynolds, Catherine; Goudet, Amélie; Jenjaroen, Kemajittra; Sumonwiriya, Manutsanun; Rinchai, Darawan; Musson, Julie; Overbeek, Saskia; Makinde, Julia; Quigley, Kathryn; Manji, Jiten; Spink, Natasha; Yos, Pagnarith; Wuthiekanun, Vanaporn; Bancroft, Gregory; Robinson, John; Lertmemongkolchai, Ganjana; Dunachie, Susanna; Maillere, Bernard; Holden, Matthew; Altmann, Daniel; Boyton, Rosemary.

In: Journal of Immunology, Vol. 194, No. 10, 01.01.2015, p. 4814-4824.

Research output: Contribution to journalArticle

Reynolds, C, Goudet, A, Jenjaroen, K, Sumonwiriya, M, Rinchai, D, Musson, J, Overbeek, S, Makinde, J, Quigley, K, Manji, J, Spink, N, Yos, P, Wuthiekanun, V, Bancroft, G, Robinson, J, Lertmemongkolchai, G, Dunachie, S, Maillere, B, Holden, M, Altmann, D & Boyton, R 2015, 'T cell immunity to the alkyl hydroperoxide reductase of Burkholderia pseudomallei: A correlate of disease outcome in acute melioidosis', Journal of Immunology, vol. 194, no. 10, pp. 4814-4824. https://doi.org/10.4049/jimmunol.1402862
Reynolds, Catherine ; Goudet, Amélie ; Jenjaroen, Kemajittra ; Sumonwiriya, Manutsanun ; Rinchai, Darawan ; Musson, Julie ; Overbeek, Saskia ; Makinde, Julia ; Quigley, Kathryn ; Manji, Jiten ; Spink, Natasha ; Yos, Pagnarith ; Wuthiekanun, Vanaporn ; Bancroft, Gregory ; Robinson, John ; Lertmemongkolchai, Ganjana ; Dunachie, Susanna ; Maillere, Bernard ; Holden, Matthew ; Altmann, Daniel ; Boyton, Rosemary. / T cell immunity to the alkyl hydroperoxide reductase of Burkholderia pseudomallei : A correlate of disease outcome in acute melioidosis. In: Journal of Immunology. 2015 ; Vol. 194, No. 10. pp. 4814-4824.
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