Synthesis and

In vitro cytotoxicity evaluation of ruthenium polypyridyl-sensitized paramagnetic titania nanoparticles for photodynamic therapy

Mohammad H. Sakr, Najeeb Halabi, Leen N. Kalash, Sara I. Al-Ghadban, Mayyasa K. Rammah, Marwan E. El Sabban, Kamal H. Bouhadir, Tarek H. Ghaddar

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Magnetite/silica/titania core-shell-shell nanoparticles were synthesized by sol-gel methods and characterized by various methods. The hydrodynamic radius of the final nanoparticle sample was measured by dynamic light scattering and found to be 99 nm. Following hydrothermal treatment of the final nanocomposites, a ruthenium polypyridyl dye was anchored to the surface. The total potential reactive oxygen species (ROS) generation and singlet oxygen (1O2) production in solution by the hybrid NPs (naked NPs + ruthenium dye) were evaluated by fluorescence and UV-Vis spectroscopy. Under 532 nm light illumination, more than a five-fold increase in ROS production was observed with the hybrid NPs in comparison to the bare NPs or the ruthenium dye alone. Similar results were obtained under white light illumination as well. Moreover, singlet oxygen generation produced by the hybrid NPs was found to be negligible. The photodynamic effect of the excited NPs on the viability of lung cancer cells (A549) was evaluated in vitro. ROS production in A549 NP-loaded cells was measured and a live/dead cell assay was conducted. There was a significant, light excitation-dependent increase in ROS production and induction of cell death in light excited A549 NP-loaded cells as compared to control cells and NP loaded cells without excitation. These findings demonstrate that the hybrid NPs can potentially act as type I photodynamic therapy (PDT) agents generating free radicals, unlike the currently employed ones in medicine which follow a type II mechanism predominantly (generating singlet oxygen). This type of photosensitizers can prove advantageous in eradicating PDT-resilient hypoxic tumors, avoiding type II photosensitizers-induced hypoxia in non-hypoxic tumor cells.

Original languageEnglish
Pages (from-to)47520-47529
Number of pages10
JournalRSC Advances
Volume6
Issue number53
DOIs
Publication statusPublished - 1 Jan 2016
Externally publishedYes

Fingerprint

Photodynamic therapy
Ruthenium
Cytotoxicity
Singlet Oxygen
Reactive Oxygen Species
Titanium
Nanoparticles
Oxygen
Photosensitizing Agents
Coloring Agents
Tumors
Lighting
Cells
Ferrosoferric Oxide
Photosensitizers
Dyes
Cell death
Dynamic light scattering
Ultraviolet spectroscopy
Silicon Dioxide

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Synthesis and : In vitro cytotoxicity evaluation of ruthenium polypyridyl-sensitized paramagnetic titania nanoparticles for photodynamic therapy. / Sakr, Mohammad H.; Halabi, Najeeb; Kalash, Leen N.; Al-Ghadban, Sara I.; Rammah, Mayyasa K.; El Sabban, Marwan E.; Bouhadir, Kamal H.; Ghaddar, Tarek H.

In: RSC Advances, Vol. 6, No. 53, 01.01.2016, p. 47520-47529.

Research output: Contribution to journalArticle

Sakr, MH, Halabi, N, Kalash, LN, Al-Ghadban, SI, Rammah, MK, El Sabban, ME, Bouhadir, KH & Ghaddar, TH 2016, 'Synthesis and: In vitro cytotoxicity evaluation of ruthenium polypyridyl-sensitized paramagnetic titania nanoparticles for photodynamic therapy', RSC Advances, vol. 6, no. 53, pp. 47520-47529. https://doi.org/10.1039/c6ra09696d
Sakr, Mohammad H. ; Halabi, Najeeb ; Kalash, Leen N. ; Al-Ghadban, Sara I. ; Rammah, Mayyasa K. ; El Sabban, Marwan E. ; Bouhadir, Kamal H. ; Ghaddar, Tarek H. / Synthesis and : In vitro cytotoxicity evaluation of ruthenium polypyridyl-sensitized paramagnetic titania nanoparticles for photodynamic therapy. In: RSC Advances. 2016 ; Vol. 6, No. 53. pp. 47520-47529.
@article{6211349d328741f1bddd5f1fef8b53c3,
title = "Synthesis and: In vitro cytotoxicity evaluation of ruthenium polypyridyl-sensitized paramagnetic titania nanoparticles for photodynamic therapy",
abstract = "Magnetite/silica/titania core-shell-shell nanoparticles were synthesized by sol-gel methods and characterized by various methods. The hydrodynamic radius of the final nanoparticle sample was measured by dynamic light scattering and found to be 99 nm. Following hydrothermal treatment of the final nanocomposites, a ruthenium polypyridyl dye was anchored to the surface. The total potential reactive oxygen species (ROS) generation and singlet oxygen (1O2) production in solution by the hybrid NPs (naked NPs + ruthenium dye) were evaluated by fluorescence and UV-Vis spectroscopy. Under 532 nm light illumination, more than a five-fold increase in ROS production was observed with the hybrid NPs in comparison to the bare NPs or the ruthenium dye alone. Similar results were obtained under white light illumination as well. Moreover, singlet oxygen generation produced by the hybrid NPs was found to be negligible. The photodynamic effect of the excited NPs on the viability of lung cancer cells (A549) was evaluated in vitro. ROS production in A549 NP-loaded cells was measured and a live/dead cell assay was conducted. There was a significant, light excitation-dependent increase in ROS production and induction of cell death in light excited A549 NP-loaded cells as compared to control cells and NP loaded cells without excitation. These findings demonstrate that the hybrid NPs can potentially act as type I photodynamic therapy (PDT) agents generating free radicals, unlike the currently employed ones in medicine which follow a type II mechanism predominantly (generating singlet oxygen). This type of photosensitizers can prove advantageous in eradicating PDT-resilient hypoxic tumors, avoiding type II photosensitizers-induced hypoxia in non-hypoxic tumor cells.",
author = "Sakr, {Mohammad H.} and Najeeb Halabi and Kalash, {Leen N.} and Al-Ghadban, {Sara I.} and Rammah, {Mayyasa K.} and {El Sabban}, {Marwan E.} and Bouhadir, {Kamal H.} and Ghaddar, {Tarek H.}",
year = "2016",
month = "1",
day = "1",
doi = "10.1039/c6ra09696d",
language = "English",
volume = "6",
pages = "47520--47529",
journal = "RSC Advances",
issn = "2046-2069",
publisher = "Royal Society of Chemistry",
number = "53",

}

TY - JOUR

T1 - Synthesis and

T2 - In vitro cytotoxicity evaluation of ruthenium polypyridyl-sensitized paramagnetic titania nanoparticles for photodynamic therapy

AU - Sakr, Mohammad H.

AU - Halabi, Najeeb

AU - Kalash, Leen N.

AU - Al-Ghadban, Sara I.

AU - Rammah, Mayyasa K.

AU - El Sabban, Marwan E.

AU - Bouhadir, Kamal H.

AU - Ghaddar, Tarek H.

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Magnetite/silica/titania core-shell-shell nanoparticles were synthesized by sol-gel methods and characterized by various methods. The hydrodynamic radius of the final nanoparticle sample was measured by dynamic light scattering and found to be 99 nm. Following hydrothermal treatment of the final nanocomposites, a ruthenium polypyridyl dye was anchored to the surface. The total potential reactive oxygen species (ROS) generation and singlet oxygen (1O2) production in solution by the hybrid NPs (naked NPs + ruthenium dye) were evaluated by fluorescence and UV-Vis spectroscopy. Under 532 nm light illumination, more than a five-fold increase in ROS production was observed with the hybrid NPs in comparison to the bare NPs or the ruthenium dye alone. Similar results were obtained under white light illumination as well. Moreover, singlet oxygen generation produced by the hybrid NPs was found to be negligible. The photodynamic effect of the excited NPs on the viability of lung cancer cells (A549) was evaluated in vitro. ROS production in A549 NP-loaded cells was measured and a live/dead cell assay was conducted. There was a significant, light excitation-dependent increase in ROS production and induction of cell death in light excited A549 NP-loaded cells as compared to control cells and NP loaded cells without excitation. These findings demonstrate that the hybrid NPs can potentially act as type I photodynamic therapy (PDT) agents generating free radicals, unlike the currently employed ones in medicine which follow a type II mechanism predominantly (generating singlet oxygen). This type of photosensitizers can prove advantageous in eradicating PDT-resilient hypoxic tumors, avoiding type II photosensitizers-induced hypoxia in non-hypoxic tumor cells.

AB - Magnetite/silica/titania core-shell-shell nanoparticles were synthesized by sol-gel methods and characterized by various methods. The hydrodynamic radius of the final nanoparticle sample was measured by dynamic light scattering and found to be 99 nm. Following hydrothermal treatment of the final nanocomposites, a ruthenium polypyridyl dye was anchored to the surface. The total potential reactive oxygen species (ROS) generation and singlet oxygen (1O2) production in solution by the hybrid NPs (naked NPs + ruthenium dye) were evaluated by fluorescence and UV-Vis spectroscopy. Under 532 nm light illumination, more than a five-fold increase in ROS production was observed with the hybrid NPs in comparison to the bare NPs or the ruthenium dye alone. Similar results were obtained under white light illumination as well. Moreover, singlet oxygen generation produced by the hybrid NPs was found to be negligible. The photodynamic effect of the excited NPs on the viability of lung cancer cells (A549) was evaluated in vitro. ROS production in A549 NP-loaded cells was measured and a live/dead cell assay was conducted. There was a significant, light excitation-dependent increase in ROS production and induction of cell death in light excited A549 NP-loaded cells as compared to control cells and NP loaded cells without excitation. These findings demonstrate that the hybrid NPs can potentially act as type I photodynamic therapy (PDT) agents generating free radicals, unlike the currently employed ones in medicine which follow a type II mechanism predominantly (generating singlet oxygen). This type of photosensitizers can prove advantageous in eradicating PDT-resilient hypoxic tumors, avoiding type II photosensitizers-induced hypoxia in non-hypoxic tumor cells.

UR - http://www.scopus.com/inward/record.url?scp=84971003308&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84971003308&partnerID=8YFLogxK

U2 - 10.1039/c6ra09696d

DO - 10.1039/c6ra09696d

M3 - Article

VL - 6

SP - 47520

EP - 47529

JO - RSC Advances

JF - RSC Advances

SN - 2046-2069

IS - 53

ER -