SWI/SNF remodeling and p300-dependent transcription of histone variant H2ABbd nucleosomal arrays

Dimitar Angelov, André Verdel, Woojin An, Vladimir Bondarenko, Fabienne Hans, Cécile Marie Doyen, Vassily M. Studitsky, Ali Hamiche, Robert G. Roeder, Philippe Bouvet, Stefan Dimitrov

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

A histone variant H2ABbd was recently identified, but its function is totally unknown. Here we have studied the structural and functional properties of nucleosome and nucleosomal arrays reconstituted with this histone variant. We show that H2ABbd can replace the conventional H2A in the nucleosome, but this replacement results in alterations of the nucleosomal structure. The remodeling complexes SWI/SNF and ACF are unable to mobilize the variant H2ABbd nucleosome. However, SWI/SNF was able to increase restriction enzyme access to the variant nucleosome and assist the transfer of variant H2ABbd-H2B dimer to a tetrameric histone H3-H4 particle. In addition, the p300- and Ga14-VP16-activated transcription appeared to be more efficient for H2ABbd nucleosomal arrays than for conventional H2A arrays. The intriguing mechanisms by which H2ABbd affects both nucleosome remodeling and transcription are discussed.

Original languageEnglish
Pages (from-to)3815-3824
Number of pages10
JournalEMBO Journal
Volume23
Issue number19
DOIs
Publication statusPublished - 1 Oct 2004
Externally publishedYes

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Nucleosomes
Transcription
Histones
Dimers
Enzymes

Keywords

  • Histone transfer
  • Histone variant H2Abbd
  • Nucleosome remodeling
  • Transcription

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

SWI/SNF remodeling and p300-dependent transcription of histone variant H2ABbd nucleosomal arrays. / Angelov, Dimitar; Verdel, André; An, Woojin; Bondarenko, Vladimir; Hans, Fabienne; Doyen, Cécile Marie; Studitsky, Vassily M.; Hamiche, Ali; Roeder, Robert G.; Bouvet, Philippe; Dimitrov, Stefan.

In: EMBO Journal, Vol. 23, No. 19, 01.10.2004, p. 3815-3824.

Research output: Contribution to journalArticle

Angelov, D, Verdel, A, An, W, Bondarenko, V, Hans, F, Doyen, CM, Studitsky, VM, Hamiche, A, Roeder, RG, Bouvet, P & Dimitrov, S 2004, 'SWI/SNF remodeling and p300-dependent transcription of histone variant H2ABbd nucleosomal arrays', EMBO Journal, vol. 23, no. 19, pp. 3815-3824. https://doi.org/10.1038/sj.emboj.7600400
Angelov, Dimitar ; Verdel, André ; An, Woojin ; Bondarenko, Vladimir ; Hans, Fabienne ; Doyen, Cécile Marie ; Studitsky, Vassily M. ; Hamiche, Ali ; Roeder, Robert G. ; Bouvet, Philippe ; Dimitrov, Stefan. / SWI/SNF remodeling and p300-dependent transcription of histone variant H2ABbd nucleosomal arrays. In: EMBO Journal. 2004 ; Vol. 23, No. 19. pp. 3815-3824.
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