Sustained Levels of FGF2 Maintain Undifferentiated Stem Cell Cultures with Biweekly Feeding

Steven Lotz, Susan Goderie, Nicolas Tokas, Sarah E. Hirsch, Faizzan Ahmad, Barbara Corneo, Sheila Le, Akhilesh Banerjee, Ravi S. Kane, Jeffrey H. Stern, Sally Temple, Christopher A. Fasano

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

An essential aspect of stem cell culture is the successful maintenance of the undifferentiated state. Many types of stem cells are FGF2 dependent, and pluripotent stem cells are maintained by replacing FGF2-containing media daily, while tissue-specific stem cells are typically fed every 3rd day. Frequent feeding, however, results in significant variation in growth factor levels due to FGF2 instability, which limits effective maintenance due to spontaneous differentiation. We report that stabilization of FGF2 levels using controlled release PLGA microspheres improves expression of stem cell markers, increases stem cell numbers and decreases spontaneous differentiation. The controlled release FGF2 additive reduces the frequency of media changes needed to maintain stem cell cultures, so that human embryonic stem cells and induced pluripotent stem cells can be maintained successfully with biweekly feedings.

Original languageEnglish
Article numbere56289
JournalPLoS One
Volume8
Issue number2
DOIs
Publication statusPublished - 20 Feb 2013
Externally publishedYes

Fingerprint

Fibroblast Growth Factor 2
Stem cells
Cell culture
stem cells
cell culture
Stem Cells
Cell Culture Techniques
Maintenance
Induced Pluripotent Stem Cells
Pluripotent Stem Cells
Microspheres
embryonic stem cells
Intercellular Signaling Peptides and Proteins
growth factors
Cell Count
Stabilization
Tissue

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Lotz, S., Goderie, S., Tokas, N., Hirsch, S. E., Ahmad, F., Corneo, B., ... Fasano, C. A. (2013). Sustained Levels of FGF2 Maintain Undifferentiated Stem Cell Cultures with Biweekly Feeding. PLoS One, 8(2), [e56289]. https://doi.org/10.1371/journal.pone.0056289

Sustained Levels of FGF2 Maintain Undifferentiated Stem Cell Cultures with Biweekly Feeding. / Lotz, Steven; Goderie, Susan; Tokas, Nicolas; Hirsch, Sarah E.; Ahmad, Faizzan; Corneo, Barbara; Le, Sheila; Banerjee, Akhilesh; Kane, Ravi S.; Stern, Jeffrey H.; Temple, Sally; Fasano, Christopher A.

In: PLoS One, Vol. 8, No. 2, e56289, 20.02.2013.

Research output: Contribution to journalArticle

Lotz, S, Goderie, S, Tokas, N, Hirsch, SE, Ahmad, F, Corneo, B, Le, S, Banerjee, A, Kane, RS, Stern, JH, Temple, S & Fasano, CA 2013, 'Sustained Levels of FGF2 Maintain Undifferentiated Stem Cell Cultures with Biweekly Feeding', PLoS One, vol. 8, no. 2, e56289. https://doi.org/10.1371/journal.pone.0056289
Lotz, Steven ; Goderie, Susan ; Tokas, Nicolas ; Hirsch, Sarah E. ; Ahmad, Faizzan ; Corneo, Barbara ; Le, Sheila ; Banerjee, Akhilesh ; Kane, Ravi S. ; Stern, Jeffrey H. ; Temple, Sally ; Fasano, Christopher A. / Sustained Levels of FGF2 Maintain Undifferentiated Stem Cell Cultures with Biweekly Feeding. In: PLoS One. 2013 ; Vol. 8, No. 2.
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