Surfactant protein-A inhibits Mycoplasma-induced dendritic cell maturation through regulation of HMGB-1 cytokine activity

Julie G. Ledford, Bernice Lo, Michele M. Kislan, Joseph M. Thomas, Katherine Evans, Derek W. Cain, Monica Kraft, Kristi L. Williams, Jo Rae Wright

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

During pulmonary infections, a careful balance between activation of protective host defense mechanisms and potentially injurious inflammatory processes must be maintained. Surfactant protein A (SP-A) is an immune modulator that increases pathogen uptake and clearance by phagocytes while minimizing lung inflammation by limiting dendritic cell (DC) and T cell activation. Recent publications have shown that SP-A binds to and is bacteriostatic for Mycoplasma pneumoniae in vitro. In vivo, SP-A aids in maintenance of airway homeostasis during M. pneumoniae pulmonary infection by preventing an overzealous proinflammatory response mediated by TNF-α. Although SP-Awas shownto inhibit maturation of DC sinvitro, the consequence of DC/SP-A interactionsinvivo has not been elucidated. In this article, weshow that the absence of SP-A during M. pneumoniae infection leads to increased numbers of mature DCs in the lung and draining lymph nodes during the acute phase of infection and, consequently, increased numbers of activated T and B cells during the course of infection. The findings that glycyrrhizin, a specific inhibitor of extracellular high-mobility group box-1 (HMGB-1) abrogated this effect and that SP-A inhibits HMGB-1 release from immune cells suggest that SP-A inhibits M. pneumoniae-induced DC maturation by regulating HMGB-1 cytokine activity.

Original languageEnglish
Pages (from-to)3884-3894
Number of pages11
JournalJournal of Immunology
Volume185
Issue number7
DOIs
Publication statusPublished - 1 Oct 2010
Externally publishedYes

Fingerprint

Pulmonary Surfactant-Associated Protein A
Mycoplasma
Dendritic Cells
Mycoplasma pneumoniae
Cytokines
Infection
Lung
Glycyrrhizic Acid
T-Lymphocytes
Phagocytes
Pneumonia
Homeostasis
B-Lymphocytes
Lymph Nodes
Maintenance

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)

Cite this

Surfactant protein-A inhibits Mycoplasma-induced dendritic cell maturation through regulation of HMGB-1 cytokine activity. / Ledford, Julie G.; Lo, Bernice; Kislan, Michele M.; Thomas, Joseph M.; Evans, Katherine; Cain, Derek W.; Kraft, Monica; Williams, Kristi L.; Wright, Jo Rae.

In: Journal of Immunology, Vol. 185, No. 7, 01.10.2010, p. 3884-3894.

Research output: Contribution to journalArticle

Ledford, JG, Lo, B, Kislan, MM, Thomas, JM, Evans, K, Cain, DW, Kraft, M, Williams, KL & Wright, JR 2010, 'Surfactant protein-A inhibits Mycoplasma-induced dendritic cell maturation through regulation of HMGB-1 cytokine activity', Journal of Immunology, vol. 185, no. 7, pp. 3884-3894. https://doi.org/10.4049/jimmunol.1000387
Ledford, Julie G. ; Lo, Bernice ; Kislan, Michele M. ; Thomas, Joseph M. ; Evans, Katherine ; Cain, Derek W. ; Kraft, Monica ; Williams, Kristi L. ; Wright, Jo Rae. / Surfactant protein-A inhibits Mycoplasma-induced dendritic cell maturation through regulation of HMGB-1 cytokine activity. In: Journal of Immunology. 2010 ; Vol. 185, No. 7. pp. 3884-3894.
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