Superresolution microscopy of the nuclear envelope and associated proteins

Wei Xie, Henning Horn, Graham D. Wright

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Citations (Scopus)

Abstract

Superresolution microscopy is undoubtedly one of the most exciting technologies since the invention of the optical microscope. Capable of nanometer-scale resolution to surpass the diffraction limit and coupled with the versatile labeling techniques available, it is revolutionizing the study of cell biology. Our understanding of the nucleus, the genetic and architectural center of the cell, has gained great advancements through the application of various superresolution microscopy techniques. This chapter describes detailed procedures of multichannel superresolution imaging of the mammalian nucleus, using structured illumination microscopy and single-molecule localization microscopy.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages83-97
Number of pages15
Volume1411
DOIs
Publication statusPublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1411
ISSN (Print)10643745

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Keywords

  • Linker of the nucleoskeleton and cytoskeleton (LINC) complex
  • Nuclear envelope (NE)
  • Nuclear lamins
  • Nuclear pore complex (NPC)
  • Photoactivated localization microscopy (PALM)
  • Singlemolecule localization microscopy (SMLM)
  • Stochastic optical reconstruction microscopy (STORM)
  • Structured illumination microscopy (SIM)
  • Superresolution microscopy (SRM)
  • Synaptonemal complex

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Xie, W., Horn, H., & Wright, G. D. (2016). Superresolution microscopy of the nuclear envelope and associated proteins. In Methods in Molecular Biology (Vol. 1411, pp. 83-97). (Methods in Molecular Biology; Vol. 1411). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3530-7_4