Superoxide-mediated actin response in post-hypoxic endothelial cells

Lawrence E. Crawford, Emily E. Milliken, Kaikobad Irani, Jay L. Zweier, Lewis C. Becker, Thomas M. Johnson, N. Tony Eissa, Ronald Crystal, Toren Finkel, Pascal J. Goldschmidt-Clermont

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

The mechanism leading to changes in the superstructure of endothelial cells exposed to ischemia and reperfusion remains uncharacterized. We show that in posthypoxic endothelial cells, the simple re-addition of oxygen induces a profound reorganization of the actin cytoskeleton. The total filamentous actin pool increases by 41% and translocation of actin filaments to the submembranous network is observed. Concurrent with the actin polymerization, increased tyrosine phosphorylation of endothelial cell substrates is detected on Western blots. Overexpression of superoxide dismutase using replication incompetent adenovirus inhibits the actin and tyrosine phosphorylation responses to reoxygenation. Inhibition of tyrosine kinases with the isoflavone genistein also suppressed the actin polymerization response to reoxygenation, but unlike superoxide dismutase, genistein also induced the collapse of the superstructure of endothelial cells upon reoxygenation. These experiments support the concept that reoxygenation following a period of hypoxia can induce the remodeling of the actin cytoskeleton in endothelial cells. Such a response requires the intact coupling of superoxide producing pathway(s) with tyrosine kinase pathway(s).

Original languageEnglish
Pages (from-to)26863-26867
Number of pages5
JournalJournal of Biological Chemistry
Volume271
Issue number43
DOIs
Publication statusPublished - 6 Nov 1996
Externally publishedYes

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Endothelial cells
Superoxides
Actins
Endothelial Cells
Actin Cytoskeleton
Genistein
Polymerization
Protein-Tyrosine Kinases
Superoxide Dismutase
Tyrosine
Phosphorylation
Isoflavones
Adenoviridae
Reperfusion
Ischemia
Western Blotting
Oxygen
Substrates

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Crawford, L. E., Milliken, E. E., Irani, K., Zweier, J. L., Becker, L. C., Johnson, T. M., ... Goldschmidt-Clermont, P. J. (1996). Superoxide-mediated actin response in post-hypoxic endothelial cells. Journal of Biological Chemistry, 271(43), 26863-26867. https://doi.org/10.1074/jbc.271.43.26863

Superoxide-mediated actin response in post-hypoxic endothelial cells. / Crawford, Lawrence E.; Milliken, Emily E.; Irani, Kaikobad; Zweier, Jay L.; Becker, Lewis C.; Johnson, Thomas M.; Tony Eissa, N.; Crystal, Ronald; Finkel, Toren; Goldschmidt-Clermont, Pascal J.

In: Journal of Biological Chemistry, Vol. 271, No. 43, 06.11.1996, p. 26863-26867.

Research output: Contribution to journalArticle

Crawford, LE, Milliken, EE, Irani, K, Zweier, JL, Becker, LC, Johnson, TM, Tony Eissa, N, Crystal, R, Finkel, T & Goldschmidt-Clermont, PJ 1996, 'Superoxide-mediated actin response in post-hypoxic endothelial cells', Journal of Biological Chemistry, vol. 271, no. 43, pp. 26863-26867. https://doi.org/10.1074/jbc.271.43.26863
Crawford LE, Milliken EE, Irani K, Zweier JL, Becker LC, Johnson TM et al. Superoxide-mediated actin response in post-hypoxic endothelial cells. Journal of Biological Chemistry. 1996 Nov 6;271(43):26863-26867. https://doi.org/10.1074/jbc.271.43.26863
Crawford, Lawrence E. ; Milliken, Emily E. ; Irani, Kaikobad ; Zweier, Jay L. ; Becker, Lewis C. ; Johnson, Thomas M. ; Tony Eissa, N. ; Crystal, Ronald ; Finkel, Toren ; Goldschmidt-Clermont, Pascal J. / Superoxide-mediated actin response in post-hypoxic endothelial cells. In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 43. pp. 26863-26867.
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