Supercritical carbon dioxide extraction of microalgae lipid

Process optimization and laboratory scale-up

Hanifa Taher, Sulaiman Al-Zuhair, Ali H. Al-Marzouqi, Yousef Haik, Mohammed Farid, Saeed Tariq

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Supercritical carbon dioxide (SC-CO2) extraction of lipid from Scenedesmus sp. for biodiesel production was investigated and compared to conventional extraction methods. The effect of biomass pre-treatment prior to extraction and extracting conditions, namely pressure in the range of 200-500 bar, temperatures in the range of 35-65 C and CO2 flow rate in the range of 1.38-4.02 g min-1, on SC-CO2 extraction yield and quality of lipid were investigated. Three levels full factorial design of experiments and response surface methodology was used to model the system. A second order polynomial model was developed and used to predict the optimum conditions. Scaling up to a laboratory larger scale was also tested. The results indicated that SC-CO2 extraction was superior to other extraction techniques, but exhibited significant variations in yield with changes in operating parameters. In the developed model, it was found that the linear and quadratic terms of the temperature, as well as the interaction with pressure had a significant effect on lipid yield; whereas, their effect on lipid quality was insignificant. The best operating conditions, in the tested range, were 53 C, 500 bar and 1.9 g min-1, in which lipid extraction yield of 7.41% (dry weight basis) was obtained. Negligible differences were observed when the fatty acid composition of SC-CO2 extracted lipid was compared to that extracted by the conventional methods. At the optimum conditions, SC-CO 2 extraction was successfully scaled-up by eight-folds and the extracted lipid yield dropped by 16%.

Original languageEnglish
Pages (from-to)57-66
Number of pages10
JournalJournal of Supercritical Fluids
Volume86
DOIs
Publication statusPublished - Feb 2014
Externally publishedYes

Fingerprint

Carbon Dioxide
Lipids
lipids
carbon dioxide
Carbon dioxide
optimization
scenedesmus
factorial design
Biofuels
experiment design
fatty acids
Carbon Monoxide
biomass
Biodiesel
Fatty acids
pretreatment
Design of experiments
polynomials
Biomass
Fatty Acids

Keywords

  • Lipid yield and quality
  • Lyophilization effect
  • Microalgae
  • Response surface methodology
  • Supercritical carbon dioxide extraction

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Chemical Engineering(all)
  • Condensed Matter Physics

Cite this

Supercritical carbon dioxide extraction of microalgae lipid : Process optimization and laboratory scale-up. / Taher, Hanifa; Al-Zuhair, Sulaiman; Al-Marzouqi, Ali H.; Haik, Yousef; Farid, Mohammed; Tariq, Saeed.

In: Journal of Supercritical Fluids, Vol. 86, 02.2014, p. 57-66.

Research output: Contribution to journalArticle

Taher, Hanifa ; Al-Zuhair, Sulaiman ; Al-Marzouqi, Ali H. ; Haik, Yousef ; Farid, Mohammed ; Tariq, Saeed. / Supercritical carbon dioxide extraction of microalgae lipid : Process optimization and laboratory scale-up. In: Journal of Supercritical Fluids. 2014 ; Vol. 86. pp. 57-66.
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