Studying the effects of semaphorins on oligodendrocyte lineage cells

Yasir Ahmed Syed, Sara Abdulla, Mark R.N. Kotter

Research output: Contribution to journalArticle

4 Citations (Scopus)


Oligodendrocytes are the myelinating cells of the central nervous system. The role of oligodendrocytes in health and disease has been considerably enhanced by the development of methods to isolate and culture oligodendrocytes from central nervous system tissue. The cellular and molecular mechanisms involved in oligodendrocyte differentiation can be identified by challenging oligodendrocyte progenitors cells (OPCs) by altering their extracellular environment and intrinsic differentiation pathways. To address these issues, it is imperative to develop an in vitro protocol where pure OPCs are isolated and cultured in the presence of inhibitory developmental and differentiation cues like Semaphorin 3A. In this chapter, we describe methods to isolate and culture OPCs from neonatal rat brain tissue and further characterise their differentiation into oligodendrocytes. The described protocol is relatively simple in comparison to existing protocols and can be used to study the effect of lesion-associated inhibitors like Semaphorin 3A on oligodendrocyte differentiation.

Original languageEnglish
Pages (from-to)363-378
Number of pages16
JournalMethods in Molecular Biology
Publication statusPublished - 1 Jan 2017
Externally publishedYes



  • Myelin
  • Neural progenitor cells
  • Oligodendrocytes
  • Semaphorin

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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