Studying dendritic cell-T cell interactions under in vivo conditions

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)


The interactions between dendritic cells and T cells during the initiation of an adaptive immune response underpins one of the most important checkpoints in ensuring that the correct immune response is induced, in order to direct the development of immune tolerance or to mount an immune response against pathogenic organisms. The advent of two-photon intravital imaging allows us to directly study the interactions between dendritic cells and T cells as they occur under physiological conditions, greatly improving our understanding of the dynamic relationship that occurs during T cell activation and expanding our knowledge of how the adaptive immune system is regulated during both priming and memory responses. Here, we describe a technique for the in vivo analysis of the interactions between either CD4+ or CD8+ T cells and dendritic cells as they occur in the popliteal lymph nodes of live mice. The adoptive transfer of both dendritic cells and T cells is utilized here in order to allow investigators to control the time point of interaction being analyzed, the activation state and amount of antigen presented by the dendritic cell, and the maturation/differentiation state of the interacting T cell.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages15
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
ISSN (Print)10643745



  • Dendritic cell
  • Immune synapse
  • Intravital
  • Popliteal lymph node
  • T cell
  • Two photon

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Van Panhuys, N. J. (2017). Studying dendritic cell-T cell interactions under in vivo conditions. In Methods in Molecular Biology (Vol. 1584, pp. 569-583). (Methods in Molecular Biology; Vol. 1584). Humana Press Inc..