Studying dendritic cell-T cell interactions under in vivo conditions

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

The interactions between dendritic cells and T cells during the initiation of an adaptive immune response underpins one of the most important checkpoints in ensuring that the correct immune response is induced, in order to direct the development of immune tolerance or to mount an immune response against pathogenic organisms. The advent of two-photon intravital imaging allows us to directly study the interactions between dendritic cells and T cells as they occur under physiological conditions, greatly improving our understanding of the dynamic relationship that occurs during T cell activation and expanding our knowledge of how the adaptive immune system is regulated during both priming and memory responses. Here, we describe a technique for the in vivo analysis of the interactions between either CD4+ or CD8+ T cells and dendritic cells as they occur in the popliteal lymph nodes of live mice. The adoptive transfer of both dendritic cells and T cells is utilized here in order to allow investigators to control the time point of interaction being analyzed, the activation state and amount of antigen presented by the dendritic cell, and the maturation/differentiation state of the interacting T cell.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages569-583
Number of pages15
Volume1584
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1584
ISSN (Print)10643745

Fingerprint

Cell Communication
Dendritic Cells
T-Lymphocytes
Immune Tolerance
Adoptive Transfer
Adaptive Immunity
Photons
Cell Differentiation
Immune System
Lymph Nodes
Research Personnel
Antigens

Keywords

  • Dendritic cell
  • Immune synapse
  • Intravital
  • Popliteal lymph node
  • T cell
  • Two photon

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Van Panhuys, N. J. (2017). Studying dendritic cell-T cell interactions under in vivo conditions. In Methods in Molecular Biology (Vol. 1584, pp. 569-583). (Methods in Molecular Biology; Vol. 1584). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6881-7_36

Studying dendritic cell-T cell interactions under in vivo conditions. / Van Panhuys, Nicholas J.

Methods in Molecular Biology. Vol. 1584 Humana Press Inc., 2017. p. 569-583 (Methods in Molecular Biology; Vol. 1584).

Research output: Chapter in Book/Report/Conference proceedingChapter

Van Panhuys, NJ 2017, Studying dendritic cell-T cell interactions under in vivo conditions. in Methods in Molecular Biology. vol. 1584, Methods in Molecular Biology, vol. 1584, Humana Press Inc., pp. 569-583. https://doi.org/10.1007/978-1-4939-6881-7_36
Van Panhuys NJ. Studying dendritic cell-T cell interactions under in vivo conditions. In Methods in Molecular Biology. Vol. 1584. Humana Press Inc. 2017. p. 569-583. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-6881-7_36
Van Panhuys, Nicholas J. / Studying dendritic cell-T cell interactions under in vivo conditions. Methods in Molecular Biology. Vol. 1584 Humana Press Inc., 2017. pp. 569-583 (Methods in Molecular Biology).
@inbook{39a16b430efb405a869a59116c1f52d6,
title = "Studying dendritic cell-T cell interactions under in vivo conditions",
abstract = "The interactions between dendritic cells and T cells during the initiation of an adaptive immune response underpins one of the most important checkpoints in ensuring that the correct immune response is induced, in order to direct the development of immune tolerance or to mount an immune response against pathogenic organisms. The advent of two-photon intravital imaging allows us to directly study the interactions between dendritic cells and T cells as they occur under physiological conditions, greatly improving our understanding of the dynamic relationship that occurs during T cell activation and expanding our knowledge of how the adaptive immune system is regulated during both priming and memory responses. Here, we describe a technique for the in vivo analysis of the interactions between either CD4+ or CD8+ T cells and dendritic cells as they occur in the popliteal lymph nodes of live mice. The adoptive transfer of both dendritic cells and T cells is utilized here in order to allow investigators to control the time point of interaction being analyzed, the activation state and amount of antigen presented by the dendritic cell, and the maturation/differentiation state of the interacting T cell.",
keywords = "Dendritic cell, Immune synapse, Intravital, Popliteal lymph node, T cell, Two photon",
author = "{Van Panhuys}, {Nicholas J.}",
year = "2017",
doi = "10.1007/978-1-4939-6881-7_36",
language = "English",
volume = "1584",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "569--583",
booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Studying dendritic cell-T cell interactions under in vivo conditions

AU - Van Panhuys, Nicholas J.

PY - 2017

Y1 - 2017

N2 - The interactions between dendritic cells and T cells during the initiation of an adaptive immune response underpins one of the most important checkpoints in ensuring that the correct immune response is induced, in order to direct the development of immune tolerance or to mount an immune response against pathogenic organisms. The advent of two-photon intravital imaging allows us to directly study the interactions between dendritic cells and T cells as they occur under physiological conditions, greatly improving our understanding of the dynamic relationship that occurs during T cell activation and expanding our knowledge of how the adaptive immune system is regulated during both priming and memory responses. Here, we describe a technique for the in vivo analysis of the interactions between either CD4+ or CD8+ T cells and dendritic cells as they occur in the popliteal lymph nodes of live mice. The adoptive transfer of both dendritic cells and T cells is utilized here in order to allow investigators to control the time point of interaction being analyzed, the activation state and amount of antigen presented by the dendritic cell, and the maturation/differentiation state of the interacting T cell.

AB - The interactions between dendritic cells and T cells during the initiation of an adaptive immune response underpins one of the most important checkpoints in ensuring that the correct immune response is induced, in order to direct the development of immune tolerance or to mount an immune response against pathogenic organisms. The advent of two-photon intravital imaging allows us to directly study the interactions between dendritic cells and T cells as they occur under physiological conditions, greatly improving our understanding of the dynamic relationship that occurs during T cell activation and expanding our knowledge of how the adaptive immune system is regulated during both priming and memory responses. Here, we describe a technique for the in vivo analysis of the interactions between either CD4+ or CD8+ T cells and dendritic cells as they occur in the popliteal lymph nodes of live mice. The adoptive transfer of both dendritic cells and T cells is utilized here in order to allow investigators to control the time point of interaction being analyzed, the activation state and amount of antigen presented by the dendritic cell, and the maturation/differentiation state of the interacting T cell.

KW - Dendritic cell

KW - Immune synapse

KW - Intravital

KW - Popliteal lymph node

KW - T cell

KW - Two photon

UR - http://www.scopus.com/inward/record.url?scp=85014623432&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85014623432&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-6881-7_36

DO - 10.1007/978-1-4939-6881-7_36

M3 - Chapter

VL - 1584

T3 - Methods in Molecular Biology

SP - 569

EP - 583

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -