Study of the diversity and short-chain fatty acids production by the bacterial community in overweight and obese Mexican children

Selvasankar Murugesan, M. Ulloa-Martínez, H. Martínez-Rojano, F. M. Galván-Rodríguez, C. Miranda-Brito, M. C. Romano, A. Piña-Escobedo, M. L. Pizano-Zárate, C. Hoyo-Vadillo, J. García-Mena

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Obesity and overweight are health problems of multifactorial etiology, which may include changes in the microbiome. In Mexico, more than 30 % of the child population between 5 and 11 years of age suffer from being overweight or are obese, which makes it a public health issue in progress. The purpose of this work was to measure the short-chain fatty acid concentration by high-performance liquid chromatography (HPLC), and to characterize the bacterial diversity by ion torrent semiconductor sequencing, of 16S rDNA libraries prepared from stools collected from a sample of well-characterized Mexican children for normal weight, overweight, and obese conditions by anthropometric and biochemical criteria. We found that triglyceride levels are increased in overweight and obese children, who presented altered propionic and butyric acid concentrations in feces. In addition, although the colon microbiota did not show a clear bacterial dysbiosis among the three conditions, the abundance of some particular bacteria was changed with respect to normal controls. We conclude from our results that the imbalance in the abundance of at least nine different bacteria as well as altered short-chain fatty acid concentration in feces is associated to the overweight and obese conditions of Mexican children.

Original languageEnglish
Pages (from-to)1337-1346
Number of pages10
JournalEuropean Journal of Clinical Microbiology and Infectious Diseases
Volume34
Issue number7
DOIs
Publication statusPublished - 23 Jul 2015
Externally publishedYes

Fingerprint

Volatile Fatty Acids
Microbiota
Feces
Dysbiosis
Bacteria
Semiconductors
Ribosomal DNA
Mexico
Colon
Triglycerides
Public Health
Obesity
High Pressure Liquid Chromatography
Ions
Weights and Measures
Health
Population

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Study of the diversity and short-chain fatty acids production by the bacterial community in overweight and obese Mexican children. / Murugesan, Selvasankar; Ulloa-Martínez, M.; Martínez-Rojano, H.; Galván-Rodríguez, F. M.; Miranda-Brito, C.; Romano, M. C.; Piña-Escobedo, A.; Pizano-Zárate, M. L.; Hoyo-Vadillo, C.; García-Mena, J.

In: European Journal of Clinical Microbiology and Infectious Diseases, Vol. 34, No. 7, 23.07.2015, p. 1337-1346.

Research output: Contribution to journalArticle

Murugesan, S, Ulloa-Martínez, M, Martínez-Rojano, H, Galván-Rodríguez, FM, Miranda-Brito, C, Romano, MC, Piña-Escobedo, A, Pizano-Zárate, ML, Hoyo-Vadillo, C & García-Mena, J 2015, 'Study of the diversity and short-chain fatty acids production by the bacterial community in overweight and obese Mexican children', European Journal of Clinical Microbiology and Infectious Diseases, vol. 34, no. 7, pp. 1337-1346. https://doi.org/10.1007/s10096-015-2355-4
Murugesan, Selvasankar ; Ulloa-Martínez, M. ; Martínez-Rojano, H. ; Galván-Rodríguez, F. M. ; Miranda-Brito, C. ; Romano, M. C. ; Piña-Escobedo, A. ; Pizano-Zárate, M. L. ; Hoyo-Vadillo, C. ; García-Mena, J. / Study of the diversity and short-chain fatty acids production by the bacterial community in overweight and obese Mexican children. In: European Journal of Clinical Microbiology and Infectious Diseases. 2015 ; Vol. 34, No. 7. pp. 1337-1346.
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