Studies into the interaction of a ferrocene-conjugates of Gly-Gly-Arg-Tyr with papain: AC voltammetry, impedance spectroscopy and surface plasmon resonance studies

Chikka N. Tharamani, Khaled Mahmoud, Ganga Ramu Vasanthakumar, Heinz Bernard Kraatz

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The interaction of papain with peptide immobilized on the Au surface altered the interfacial electron transfer resistant, charge transfer resistance (RCT), by preventing the redox species approaching the electrode was characterized by electrochemical impedance spectroscopy (EIS), AC voltammetry, cyclic voltammetry and surface plasmon resonance techniques (SPR). From cyclic voltammetry a potential shift of 25-30 mV was observed after the addition of papain, with decrease in current and the impedance results showed that RCT increased with increasing concentration of papain indicating the interaction of papain with the immobilized film. This change in kinetics was also observed in SPR. The interaction was further confirmed by reflection-absorption infrared spectroscopy (RAIR).

Original languageEnglish
Pages (from-to)253-258
Number of pages6
JournalSensors and Actuators, B: Chemical
Volume137
Issue number1
DOIs
Publication statusPublished - 28 Mar 2009
Externally publishedYes

    Fingerprint

Keywords

  • Electrochemical impedance spectroscopy
  • Papain
  • Peptides
  • Sensors
  • SPR

ASJC Scopus subject areas

  • Instrumentation
  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Electrical and Electronic Engineering

Cite this