Structure of liganded T-state haemoglobin from cat (Felis silvestris catus), a low oxygen-affinity species, in two different crystal forms

Balasubramanian Moovarkumudalvan, Ponnuraj Sathya Moorthy, Kamariah Neelagandan, Ramya Ramadoss, Prasanna Kolatkar, M. N. Ponnuswamy

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Haemoglobin (Hb) is an iron-containing metalloprotein which plays a major role in the transportation of oxygen from the lungs to tissues and of carbon dioxide back to the lungs. Hb is in equilibrium between low-affinity tense (T) and high-affinity relaxed (R) states associated with its unliganded and liganded forms, respectively. Mammalian species can be classified into two groups on the basis of whether they express 'high' or 'low' oxygen-affinity Hbs. Although Hbs from the former group have been studied extensively, a more limited number of structural studies have been performed for low oxygen-affinity Hbs. Here, the crystal structure of low oxygen-affinity cat methaemoglobin (metHb) has been solved at 2.0 and 2.4 Å resolution in two different crystal forms. Even though both structures are fully liganded, they unusually adopt a T-state-like quaternary conformation but with several localized R-like tertiary-structural and quaternary-structural features. The study provides atomic-level insights into the ligand-binding properties of this Hb, including its low cooperativity, blunt response to allosteric effectors and low affinity for oxygen, as well as further contributing to the mechanism underlying Hb allostery.

Original languageEnglish
Pages (from-to)1898-1906
Number of pages9
JournalActa Crystallographica Section D: Biological Crystallography
Volume70
Issue number7
DOIs
Publication statusPublished - 1 Jan 2014

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Cats
Oxygen
Hemoglobins
Metalloproteins
Methemoglobin
Lung
Carbon Dioxide
Iron
deoxyhemoglobin
Ligands

Keywords

  • 2,3-diphosphoglycerate
  • allosteric mechanism
  • cat
  • cooperativity
  • Felis silvestris catus
  • haemoglobin
  • liganded T-state
  • low oxygen affinity

ASJC Scopus subject areas

  • Structural Biology

Cite this

Structure of liganded T-state haemoglobin from cat (Felis silvestris catus), a low oxygen-affinity species, in two different crystal forms. / Moovarkumudalvan, Balasubramanian; Moorthy, Ponnuraj Sathya; Neelagandan, Kamariah; Ramadoss, Ramya; Kolatkar, Prasanna; Ponnuswamy, M. N.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. 70, No. 7, 01.01.2014, p. 1898-1906.

Research output: Contribution to journalArticle

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