Structural studies on a low oxygen affinity hemoglobin from mammalian species

Sheep (Ovis aries)

Neelagandan Kamariah, Sathya Moorthy Ponnuraj, Balasubramanian Moovarkumudalvan, Mondikalipudur Nanjappa Gounder Ponnuswamy

Research output: Contribution to journalArticle

Abstract

Hemoglobin (Hb) is in equilibrium between low affinity Tense (T) and high affinity Relaxed (R) states associated with its unliganded and liganded forms, respectively. Mammalian species can be classified into two groups on the basis of whether they express 'high' and 'low' oxygen affinity Hbs. Although Hbs from former group have been studied extensively, a limited number of structural studies have been performed for the low oxygen affinity Hbs. Here, the crystal structure of low oxygen affinity sheep methemoglobin (metHb) has been determined to 2.7 Å resolution. Even though sheep metHb adopts classical R state like quaternary structure, it shows localized quaternary and tertiary structural differences compared with other liganded Hb. The critical group of residues in the "joint region", shown as a major source of quaternary constraint on deoxyHb, formed unique interactions in the α1β2/α2β1 interfaces of sheep metHb structure. In addition, the constrained β subunits heme environment and the contraction of N-termini and A-helices of β subunits towards the molecular dyad are observed for sheep metHb structure. These observations provide the structural basis for a low oxygen affinity and blunt response to allosteric effector of sheep Hb.

Original languageEnglish
Pages (from-to)36-41
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume450
Issue number1
DOIs
Publication statusPublished - 18 Jul 2014

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Domestic Sheep
Methemoglobin
Sheep
Hemoglobins
Oxygen
Heme
Crystal structure
Joints

Keywords

  • Allosteric mechanism
  • Crystal structure
  • Hemoglobin
  • Low oxygen affinity
  • Sheep

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Structural studies on a low oxygen affinity hemoglobin from mammalian species : Sheep (Ovis aries). / Kamariah, Neelagandan; Ponnuraj, Sathya Moorthy; Moovarkumudalvan, Balasubramanian; Ponnuswamy, Mondikalipudur Nanjappa Gounder.

In: Biochemical and Biophysical Research Communications, Vol. 450, No. 1, 18.07.2014, p. 36-41.

Research output: Contribution to journalArticle

Kamariah, Neelagandan ; Ponnuraj, Sathya Moorthy ; Moovarkumudalvan, Balasubramanian ; Ponnuswamy, Mondikalipudur Nanjappa Gounder. / Structural studies on a low oxygen affinity hemoglobin from mammalian species : Sheep (Ovis aries). In: Biochemical and Biophysical Research Communications. 2014 ; Vol. 450, No. 1. pp. 36-41.
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