Structural studies of two rhinovirus serotypes complexed with fragments of their cellular receptor

Prasanna Kolatkar, Jordi Bella, Norman H. Olson, Carol M. Bator, Timothy S. Baker, Michael G. Rossmann

Research output: Contribution to journalArticle

149 Citations (Scopus)

Abstract

Two human rhinovirus serotypes complexed with two- and five-domain soluble fragments of the cellular receptor, intercellular adhesion molecule-1, have been investigated by X-ray crystallographic analyses of the individual components and by cryo-electron microscopy of the complexes. The three-dimensional image reconstructions provide a molecular envelope within which the crystal structures of the viruses and the receptor fragments can be positioned with accuracy. The N-terminal domain of the receptor binds to the rhinovirus 'canyon' surrounding the icosahedral 5-fold axes. Fitting of molecular models into the image reconstruction density identified the residues on the virus that interact with those on the receptor surface, demonstrating complementarity of the electrostatic patterns for the tip of the N-terminal receptor domain and the floor of the canyon. The complexes seen in the image reconstructions probably represent the first stage of a multistep binding process. A mechanism is proposed for the subsequent viral uncoating process.

Original languageEnglish
Pages (from-to)6249-6259
Number of pages11
JournalEMBO Journal
Volume18
Issue number22
DOIs
Publication statusPublished - 15 Nov 1999
Externally publishedYes

Fingerprint

Rhinovirus
Computer-Assisted Image Processing
Image reconstruction
Virus Uncoating
Virus Receptors
Cryoelectron Microscopy
Molecular Models
Three-Dimensional Imaging
Intercellular Adhesion Molecule-1
Static Electricity
Viruses
Electron microscopy
Electrostatics
Crystal structure
X-Rays
X rays
Serogroup

Keywords

  • Cryo-EM
  • Crystallography
  • ICAM-1
  • Receptor specificity
  • Rhinoviruses

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Structural studies of two rhinovirus serotypes complexed with fragments of their cellular receptor. / Kolatkar, Prasanna; Bella, Jordi; Olson, Norman H.; Bator, Carol M.; Baker, Timothy S.; Rossmann, Michael G.

In: EMBO Journal, Vol. 18, No. 22, 15.11.1999, p. 6249-6259.

Research output: Contribution to journalArticle

Kolatkar, Prasanna ; Bella, Jordi ; Olson, Norman H. ; Bator, Carol M. ; Baker, Timothy S. ; Rossmann, Michael G. / Structural studies of two rhinovirus serotypes complexed with fragments of their cellular receptor. In: EMBO Journal. 1999 ; Vol. 18, No. 22. pp. 6249-6259.
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