Structural genomic abnormalities of chromosomes 9 and 18 in myxopapillary ependymomas

Maria Betania Mahler-Araujo, Despina Sanoudou, Ola Tingby, Lu Liu, Nicholas Coleman, Koichi Ichimura, Vincent Peter Collins

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Myxopapillary ependymomas (MPEs) are low-grade neuroepithelial tumors typically occurring in the conus - cauda equina - filum terminale region. Limited molecular and cytogenetic analysis of MPEs has not demonstrated consistent abnormalities. In an attempt to clarify the chromosomal status of these tumors and identify commonly aberrant regions in the genome we have combined 3 molecular/cyto/genetic methods to study 17 MPEs. Comparative genomic hybridization of 7/17 tumors identified concurrent gain on chromosomes 9 and 18 as the most frequent finding. The majority of the 17 tumors were also studied using microsatellite analysis with marker spanning the whole chromosomes 9 and 18 and interphase-FISH with centromeric probes for both chromosomes. Our combined results were consistent with concurrent gain in both chromosomes 9 and 18 in 11/17 cases, gain of either chromosome 9 or 18 and imbalance in the other chromosome in 3/17 tumors and allelic imbalances of chromosomes 9 or 18 in 3/17 and 1/17 tumors, respectively. Other abnormalities observed included gain of chromosomes 3, 4, 7, 8, 11, 13, 17q, 20, and X and loss of chromosomes 10 and 22. Our findings represent some steps towards understanding the molecular mechanisms involved in the development of MPE.

Original languageEnglish
Pages (from-to)927-935
Number of pages9
JournalJournal of Neuropathology and Experimental Neurology
Volume62
Issue number9
Publication statusPublished - 1 Sep 2003
Externally publishedYes

Fingerprint

Chromosomes, Human, Pair 18
Ependymoma
Chromosomes, Human, Pair 9
Cauda Equina
Chromosomes, Human, Pair 3
Neoplasms
Allelic Imbalance
Neuroepithelial Neoplasms
Chromosomes, Human, Pair 22
Chromosomes, Human, Pair 10
Chromosomes, Human, Pair 4
Comparative Genomic Hybridization
Cytogenetic Analysis
Interphase
X Chromosome
Microsatellite Repeats
Molecular Biology
Chromosomes
Genome

Keywords

  • Aneusomy
  • Central nervous system tumors
  • Comparative genomic hybridization
  • Fluorescence in situ hybridization
  • Microsatellite analysis
  • Polysomy

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Neuroscience(all)

Cite this

Mahler-Araujo, M. B., Sanoudou, D., Tingby, O., Liu, L., Coleman, N., Ichimura, K., & Collins, V. P. (2003). Structural genomic abnormalities of chromosomes 9 and 18 in myxopapillary ependymomas. Journal of Neuropathology and Experimental Neurology, 62(9), 927-935.

Structural genomic abnormalities of chromosomes 9 and 18 in myxopapillary ependymomas. / Mahler-Araujo, Maria Betania; Sanoudou, Despina; Tingby, Ola; Liu, Lu; Coleman, Nicholas; Ichimura, Koichi; Collins, Vincent Peter.

In: Journal of Neuropathology and Experimental Neurology, Vol. 62, No. 9, 01.09.2003, p. 927-935.

Research output: Contribution to journalArticle

Mahler-Araujo, MB, Sanoudou, D, Tingby, O, Liu, L, Coleman, N, Ichimura, K & Collins, VP 2003, 'Structural genomic abnormalities of chromosomes 9 and 18 in myxopapillary ependymomas', Journal of Neuropathology and Experimental Neurology, vol. 62, no. 9, pp. 927-935.
Mahler-Araujo MB, Sanoudou D, Tingby O, Liu L, Coleman N, Ichimura K et al. Structural genomic abnormalities of chromosomes 9 and 18 in myxopapillary ependymomas. Journal of Neuropathology and Experimental Neurology. 2003 Sep 1;62(9):927-935.
Mahler-Araujo, Maria Betania ; Sanoudou, Despina ; Tingby, Ola ; Liu, Lu ; Coleman, Nicholas ; Ichimura, Koichi ; Collins, Vincent Peter. / Structural genomic abnormalities of chromosomes 9 and 18 in myxopapillary ependymomas. In: Journal of Neuropathology and Experimental Neurology. 2003 ; Vol. 62, No. 9. pp. 927-935.
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