Structural Characterization of MacroH2A Containing Chromatin

D. Wade Abbott, Mario Laszczak, John D. Lewis, Harvey Su, Susan C. Moore, Melissa Hills, Stefan Dimitrov, Juan Ausió

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

MacroH2A (mH2A) is one of the most recently identified members of the heteromorphous histone variant family. It is unique among the members of this group because it contains an unusually large non-histone C-terminal end, from where its name derives, and appears to be restricted to subphylum vertebrata. Although a concerted effort has been carried out in order to characterize the physiological relevance of mH2A, little is known in comparison about the structural importance of the molecule. Elucidating the biophysical and conformational proprieties of mH2A in chromatin may provide clues into the links between this histone variant and its unique function(s). In this paper, we look first at the heterogeneous tissue-specific distribution of this protein in different vertebrate classes. This is followed by a structural comparison between mH2A and H2A protein and by the characterization of the nucleosome core particles with which these histone subtypes are associated. We find that the highly α-helical C-terminus of mH2A confers an asymmetric conformation to nucleosomes and that this variant is tightly bound to chromatin fragments in a way that does not depend on the overall extent of acetylation of the other core histones.

Original languageEnglish
Pages (from-to)1352-1359
Number of pages8
JournalBiochemistry
Volume43
Issue number5
DOIs
Publication statusPublished - 10 Feb 2004
Externally publishedYes

Fingerprint

Histones
Chromatin
Nucleosomes
Acetylation
Tissue Distribution
Names
Conformations
Vertebrates
Proteins
Tissue
Molecules

ASJC Scopus subject areas

  • Biochemistry

Cite this

Abbott, D. W., Laszczak, M., Lewis, J. D., Su, H., Moore, S. C., Hills, M., ... Ausió, J. (2004). Structural Characterization of MacroH2A Containing Chromatin. Biochemistry, 43(5), 1352-1359. https://doi.org/10.1021/bi035859i

Structural Characterization of MacroH2A Containing Chromatin. / Abbott, D. Wade; Laszczak, Mario; Lewis, John D.; Su, Harvey; Moore, Susan C.; Hills, Melissa; Dimitrov, Stefan; Ausió, Juan.

In: Biochemistry, Vol. 43, No. 5, 10.02.2004, p. 1352-1359.

Research output: Contribution to journalArticle

Abbott, DW, Laszczak, M, Lewis, JD, Su, H, Moore, SC, Hills, M, Dimitrov, S & Ausió, J 2004, 'Structural Characterization of MacroH2A Containing Chromatin', Biochemistry, vol. 43, no. 5, pp. 1352-1359. https://doi.org/10.1021/bi035859i
Abbott DW, Laszczak M, Lewis JD, Su H, Moore SC, Hills M et al. Structural Characterization of MacroH2A Containing Chromatin. Biochemistry. 2004 Feb 10;43(5):1352-1359. https://doi.org/10.1021/bi035859i
Abbott, D. Wade ; Laszczak, Mario ; Lewis, John D. ; Su, Harvey ; Moore, Susan C. ; Hills, Melissa ; Dimitrov, Stefan ; Ausió, Juan. / Structural Characterization of MacroH2A Containing Chromatin. In: Biochemistry. 2004 ; Vol. 43, No. 5. pp. 1352-1359.
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