Structural and functional consequence of neonatal sympathectomy on the blood vessels of spontaneously hypertensive rats

Robert M K W Lee, Christopher Triggle, Donald W T Cheung, Michael D. Coughlin

Research output: Contribution to journalArticle

128 Citations (Scopus)

Abstract

Neonatal sympathectomy of spontaneously hypertensive rats (SHR) and control Wistar-Kyoto rats (WKY) was performed by a combined treatment with antiserum to nerve growth factor and guanethidine during the first 4 weeks after birth. The development of hypertension was completely prevented in the treated SHR: at 28 to 30 weeks of age, systolic blood pressure of treated SHR was 139 ± 2 mm Hg as compared with 195 ± 8 mm Hg in untreated SHR. The extent of sympathectomy was verified by histofluorescence. Fluorescence histochemistry for catecholamine-containing nerves showed a complete absence of adrenergic nerves in the mesenteric arteries of treated rats. A supersensitivity to norepinephrine was exhibited by mesenteric arteries, anococcygeus muscle, and tail arteries from the treated SHR and WKY. In the mesenteric vascular bed, maximal response to norepinephrine was significantly reduced by sympathectomy. Sympathectomy also abolished the responses (e.g., generation of excitatory junctional potentials) of tail arteries to electrical stimulation of perivascular nerves. Morphometric measurements of three categories of mesenteric arteries showed that sympathectomy had no effect on the hypertrophic change of smooth muscle cells in the conducting vessels, but it prevented the hyperplastic changes of the muscle cells from reactive, muscular arteries and small resistance vessels. These results suggest that one of the primary roles of the overactive sympathetic nervous system in the development of hypertension in SHR is manifested through its trophic effect on the arteries of SHR. This trophic effect appears to cause a hyperplastic change in the smooth muscle cells in the reactive and resistance vessels, thereby contributing to the development of hypertension in older SHR.

Original languageEnglish
Pages (from-to)328-338
Number of pages11
JournalHypertension
Volume10
Issue number3
Publication statusPublished - 1987
Externally publishedYes

Fingerprint

Sympathectomy
Inbred SHR Rats
Blood Vessels
Mesenteric Arteries
Arteries
Inbred WKY Rats
Hypertension
Smooth Muscle Myocytes
Tail
Norepinephrine
Blood Pressure
Guanethidine
Sympathetic Nervous System
Nerve Growth Factor
Adrenergic Agents
Muscle Cells
Electric Stimulation
Catecholamines
Immune Sera
Fluorescence

Keywords

  • Arteries
  • Guanethidine
  • Hypertension
  • Nerve growth factor
  • Spontaneously hypertensive rats
  • Sympathetic nervous system

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Structural and functional consequence of neonatal sympathectomy on the blood vessels of spontaneously hypertensive rats. / Lee, Robert M K W; Triggle, Christopher; Cheung, Donald W T; Coughlin, Michael D.

In: Hypertension, Vol. 10, No. 3, 1987, p. 328-338.

Research output: Contribution to journalArticle

Lee, Robert M K W ; Triggle, Christopher ; Cheung, Donald W T ; Coughlin, Michael D. / Structural and functional consequence of neonatal sympathectomy on the blood vessels of spontaneously hypertensive rats. In: Hypertension. 1987 ; Vol. 10, No. 3. pp. 328-338.
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