Store-operated calcium entry inactivates at the germinal vesicle breakdown stage of Xenopus meiosis

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Abstract

Store-operated calcium entry (SOCE) is the predominant Ca2+ influx pathway in non-excitable cells and is activated in response to depletion of intracellular Ca2+ stores. We have studied SOCE regulation during Xenopus oocyte meiosis. SOCE can be measured readily in stage VI Xenopus oocytes arrested at the G2-M transition of the cell cycle, either by Ca2+ imaging or by recording the SOCE current. However, following meiotic maturation, SOCE can no longer be activated by store depletion. We have characterized the time course of SOCE inactivation during oocyte maturation, and show that SOCE inactivates almost completely, in a very short time period, at the germinal vesicle breakdown stage of meiosis. This acute inactivation offers an opportunity to better understand SOCE regulation.

Original languageEnglish
Pages (from-to)38710-38715
Number of pages6
JournalJournal of Biological Chemistry
Volume275
Issue number49
DOIs
Publication statusPublished - 8 Dec 2000
Externally publishedYes

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Meiosis
Xenopus
Calcium
Oocytes
Cell Cycle
Cells
Imaging techniques

ASJC Scopus subject areas

  • Biochemistry

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Store-operated calcium entry inactivates at the germinal vesicle breakdown stage of Xenopus meiosis. / Machaca, Khaled; Haun, S.

In: Journal of Biological Chemistry, Vol. 275, No. 49, 08.12.2000, p. 38710-38715.

Research output: Contribution to journalArticle

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