Stimuli-responsive organization of block copolymers on DNA nanotubes

Karina M M Carneiro, Graham D. Hamblin, Kevin D. Hänni, Johans Fakhoury, Manoj K. Nayak, Georgios Rizis, Christopher K. McLaughlin, Hassan S. Bazzi, Hanadi F. Sleiman

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

DNA nanotubes are an attractive class of materials that can be assembled with precise control over their size, shape, length and porosity, and can encapsulate and release materials in response to specific added molecules. In parallel, block copolymer assemblies can provide biocompatibility, stability, nuclease resistance, the ability to encapsulate small molecules, long-range assembly and numerous additional functionalities that can be tuned with subtle chemical modifications. Herein, we describe a new class of hybrid materials in which block copolymer assemblies are sequence-specifically and longitudinally positioned on robust DNA nanotubes constructed using rolling circle amplification. These materials are dynamic, allowing the polymer structures to be cleanly removed from the DNA nanotubes only when a specific DNA sequence is added, and creating a single-stranded form of these nanotubes. Specifically, we first describe the use of rolling circle amplification to create a long DNA strand containing a repeat sequence. This is used as a guide strand in a new method to construct robust nanotubes with a non-nicked backbone. We then synthesize polymer-DNA conjugates through on-column functionalization of a DNA strand with a polymer. These conjugates self-assemble into spherical aggregates, which then position themselves onto the DNA nanotubes using sequence-specific hybridization, creating a 'striped' structure. The polymer aggregates can be cleanly lifted off the nanotubes using a strand displacement strategy, thus uncapping these DNA nanotubes. We also show that this longitudinal alignment of polymer aggregates on DNA nanotubes is general for a variety of DNA-block copolymers.

Original languageEnglish
Pages (from-to)1980-1986
Number of pages7
JournalChemical Science
Volume3
Issue number6
DOIs
Publication statusPublished - Jun 2012

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Nanotubes
Block copolymers
DNA
Polymers
Amplification
Molecules
DNA sequences
Hybrid materials
Chemical modification
Biocompatibility
Porosity

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Carneiro, K. M. M., Hamblin, G. D., Hänni, K. D., Fakhoury, J., Nayak, M. K., Rizis, G., ... Sleiman, H. F. (2012). Stimuli-responsive organization of block copolymers on DNA nanotubes. Chemical Science, 3(6), 1980-1986. https://doi.org/10.1039/c2sc01065h

Stimuli-responsive organization of block copolymers on DNA nanotubes. / Carneiro, Karina M M; Hamblin, Graham D.; Hänni, Kevin D.; Fakhoury, Johans; Nayak, Manoj K.; Rizis, Georgios; McLaughlin, Christopher K.; Bazzi, Hassan S.; Sleiman, Hanadi F.

In: Chemical Science, Vol. 3, No. 6, 06.2012, p. 1980-1986.

Research output: Contribution to journalArticle

Carneiro, KMM, Hamblin, GD, Hänni, KD, Fakhoury, J, Nayak, MK, Rizis, G, McLaughlin, CK, Bazzi, HS & Sleiman, HF 2012, 'Stimuli-responsive organization of block copolymers on DNA nanotubes', Chemical Science, vol. 3, no. 6, pp. 1980-1986. https://doi.org/10.1039/c2sc01065h
Carneiro KMM, Hamblin GD, Hänni KD, Fakhoury J, Nayak MK, Rizis G et al. Stimuli-responsive organization of block copolymers on DNA nanotubes. Chemical Science. 2012 Jun;3(6):1980-1986. https://doi.org/10.1039/c2sc01065h
Carneiro, Karina M M ; Hamblin, Graham D. ; Hänni, Kevin D. ; Fakhoury, Johans ; Nayak, Manoj K. ; Rizis, Georgios ; McLaughlin, Christopher K. ; Bazzi, Hassan S. ; Sleiman, Hanadi F. / Stimuli-responsive organization of block copolymers on DNA nanotubes. In: Chemical Science. 2012 ; Vol. 3, No. 6. pp. 1980-1986.
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