Stimulation of transforming growth factor-β1 transcription by cyclosporine

Y. Prashar, A. Khanna, P. Sehajpal, V. K. Sharma, M. Suthanthiran

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

In searching for a candidate mechanism for the immunosuppressive as well as fibrogenic consequences of cyclosporine usage, we have explored the hypothesis that cyclosporine stimulates transcription of transforming growth factor-β1 (TGF-β1), a multifunctional cytokine endowed with immunosuppressive and fibrogenic properties. Our results demonstrate that cyclosporine (i) stimulates TGF-β1 promoter-dependent transcription of chloramphenicol acetyl transferase gene in transiently transfected human A-549 cells, (ii) stimulates the synthesis of TGF-β1 RNA transcripts in human T cells, and (iii) permits the expression/emergence of DNA regulatory proteins (retinoblastoma control factor-1 (RCF-1) and RCF-2) that bind and regulate TGF-β1 promoter activity. Our studies demonstrate for the first time that cyclosporine stimulates TGF-β1 gene transcription and suggest a novel mechanism of action of cyclosporine.

Original languageEnglish
Pages (from-to)109-112
Number of pages4
JournalFEBS Letters
Volume358
Issue number2
DOIs
Publication statusPublished - 23 Jan 1995

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Keywords

  • CAT activity
  • Cyclosporine
  • Mobility shift assay
  • TGF-β
  • Transcription
  • Transcription factor

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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