Stimulation of transforming growth factor-β1 transcription by cyclosporine

Y. Prashar, A. Khanna, P. Sehajpal, V. K. Sharma, Manikkam Suthanthiran

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

In searching for a candidate mechanism for the immunosuppressive as well as fibrogenic consequences of cyclosporine usage, we have explored the hypothesis that cyclosporine stimulates transcription of transforming growth factor-β1 (TGF-β1), a multifunctional cytokine endowed with immunosuppressive and fibrogenic properties. Our results demonstrate that cyclosporine (i) stimulates TGF-β1 promoter-dependent transcription of chloramphenicol acetyl transferase gene in transiently transfected human A-549 cells, (ii) stimulates the synthesis of TGF-β1 RNA transcripts in human T cells, and (iii) permits the expression/emergence of DNA regulatory proteins (retinoblastoma control factor-1 (RCF-1) and RCF-2) that bind and regulate TGF-β1 promoter activity. Our studies demonstrate for the first time that cyclosporine stimulates TGF-β1 gene transcription and suggest a novel mechanism of action of cyclosporine.

Original languageEnglish
Pages (from-to)109-112
Number of pages4
JournalFEBS Letters
Volume358
Issue number2
DOIs
Publication statusPublished - 23 Jan 1995
Externally publishedYes

Fingerprint

Transforming Growth Factors
Transcription
Cyclosporine
Immunosuppressive Agents
Genes
Retinoblastoma Protein
T-cells
Chloramphenicol
Transferases
RNA
Cytokines
T-Lymphocytes
DNA

Keywords

  • CAT activity
  • Cyclosporine
  • Mobility shift assay
  • TGF-β
  • Transcription
  • Transcription factor

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Stimulation of transforming growth factor-β1 transcription by cyclosporine. / Prashar, Y.; Khanna, A.; Sehajpal, P.; Sharma, V. K.; Suthanthiran, Manikkam.

In: FEBS Letters, Vol. 358, No. 2, 23.01.1995, p. 109-112.

Research output: Contribution to journalArticle

Prashar, Y. ; Khanna, A. ; Sehajpal, P. ; Sharma, V. K. ; Suthanthiran, Manikkam. / Stimulation of transforming growth factor-β1 transcription by cyclosporine. In: FEBS Letters. 1995 ; Vol. 358, No. 2. pp. 109-112.
@article{78bb6862107f43c8972e0a6553e6e245,
title = "Stimulation of transforming growth factor-β1 transcription by cyclosporine",
abstract = "In searching for a candidate mechanism for the immunosuppressive as well as fibrogenic consequences of cyclosporine usage, we have explored the hypothesis that cyclosporine stimulates transcription of transforming growth factor-β1 (TGF-β1), a multifunctional cytokine endowed with immunosuppressive and fibrogenic properties. Our results demonstrate that cyclosporine (i) stimulates TGF-β1 promoter-dependent transcription of chloramphenicol acetyl transferase gene in transiently transfected human A-549 cells, (ii) stimulates the synthesis of TGF-β1 RNA transcripts in human T cells, and (iii) permits the expression/emergence of DNA regulatory proteins (retinoblastoma control factor-1 (RCF-1) and RCF-2) that bind and regulate TGF-β1 promoter activity. Our studies demonstrate for the first time that cyclosporine stimulates TGF-β1 gene transcription and suggest a novel mechanism of action of cyclosporine.",
keywords = "CAT activity, Cyclosporine, Mobility shift assay, TGF-β, Transcription, Transcription factor",
author = "Y. Prashar and A. Khanna and P. Sehajpal and Sharma, {V. K.} and Manikkam Suthanthiran",
year = "1995",
month = "1",
day = "23",
doi = "10.1016/0014-5793(94)01382-B",
language = "English",
volume = "358",
pages = "109--112",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Stimulation of transforming growth factor-β1 transcription by cyclosporine

AU - Prashar, Y.

AU - Khanna, A.

AU - Sehajpal, P.

AU - Sharma, V. K.

AU - Suthanthiran, Manikkam

PY - 1995/1/23

Y1 - 1995/1/23

N2 - In searching for a candidate mechanism for the immunosuppressive as well as fibrogenic consequences of cyclosporine usage, we have explored the hypothesis that cyclosporine stimulates transcription of transforming growth factor-β1 (TGF-β1), a multifunctional cytokine endowed with immunosuppressive and fibrogenic properties. Our results demonstrate that cyclosporine (i) stimulates TGF-β1 promoter-dependent transcription of chloramphenicol acetyl transferase gene in transiently transfected human A-549 cells, (ii) stimulates the synthesis of TGF-β1 RNA transcripts in human T cells, and (iii) permits the expression/emergence of DNA regulatory proteins (retinoblastoma control factor-1 (RCF-1) and RCF-2) that bind and regulate TGF-β1 promoter activity. Our studies demonstrate for the first time that cyclosporine stimulates TGF-β1 gene transcription and suggest a novel mechanism of action of cyclosporine.

AB - In searching for a candidate mechanism for the immunosuppressive as well as fibrogenic consequences of cyclosporine usage, we have explored the hypothesis that cyclosporine stimulates transcription of transforming growth factor-β1 (TGF-β1), a multifunctional cytokine endowed with immunosuppressive and fibrogenic properties. Our results demonstrate that cyclosporine (i) stimulates TGF-β1 promoter-dependent transcription of chloramphenicol acetyl transferase gene in transiently transfected human A-549 cells, (ii) stimulates the synthesis of TGF-β1 RNA transcripts in human T cells, and (iii) permits the expression/emergence of DNA regulatory proteins (retinoblastoma control factor-1 (RCF-1) and RCF-2) that bind and regulate TGF-β1 promoter activity. Our studies demonstrate for the first time that cyclosporine stimulates TGF-β1 gene transcription and suggest a novel mechanism of action of cyclosporine.

KW - CAT activity

KW - Cyclosporine

KW - Mobility shift assay

KW - TGF-β

KW - Transcription

KW - Transcription factor

UR - http://www.scopus.com/inward/record.url?scp=0028817544&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028817544&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(94)01382-B

DO - 10.1016/0014-5793(94)01382-B

M3 - Article

C2 - 7828718

AN - SCOPUS:0028817544

VL - 358

SP - 109

EP - 112

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 2

ER -