Standardless identification of selenocystathionine and its γ-glutamyl derivatives in monkeypot nuts by 3D liquid chromatography with ICP-MS detection followed by nanoHPLC-Q-TOF-MS/MS

Mihaly Dernovics, Tamara García-Barrera, Katarzyna Bierla, Hugues Preud'homme, Ryszard Lobinski

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

A three-step chromatographic procedure using orthogonal separation mechanisms (size-exclusion, cation-exchange and ion-pairing reversed phase) was developed to purify three low molecular weight selenospecies, including the major compound, from the aqueous extract of monkeypot (Lecythis minor) nuts. The following reversed-phase nanoHPLC-electrospray Q-TOF-MS/MS allowed the formal standardless identification of selenocystathionine and two isoforms of γ-glutamyl-selenocystathionine. This is the first MS and MS/MS-based formal evidence of the presence of these compounds in a biological sample.

Original languageEnglish
Pages (from-to)439-449
Number of pages11
JournalAnalyst
Volume132
Issue number5
DOIs
Publication statusPublished - 8 May 2007
Externally publishedYes

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Nuts
nut
Liquid chromatography
Liquid Chromatography
liquid chromatography
ion exchange
Ion exchange
Positive ions
Molecular weight
Derivatives
ion
Ion Exchange
Ions
Cations
Protein Isoforms
Molecular Weight
selenocystathionine
detection

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Standardless identification of selenocystathionine and its γ-glutamyl derivatives in monkeypot nuts by 3D liquid chromatography with ICP-MS detection followed by nanoHPLC-Q-TOF-MS/MS. / Dernovics, Mihaly; García-Barrera, Tamara; Bierla, Katarzyna; Preud'homme, Hugues; Lobinski, Ryszard.

In: Analyst, Vol. 132, No. 5, 08.05.2007, p. 439-449.

Research output: Contribution to journalArticle

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