Standardless identification of selenocystathionine and its γ-glutamyl derivatives in monkeypot nuts by 3D liquid chromatography with ICP-MS detection followed by nanoHPLC-Q-TOF-MS/MS

Mihaly Dernovics, Tamara García-Barrera, Katarzyna Bierla, Hugues Preud'homme, Ryszard Lobinski

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29 Citations (Scopus)

Abstract

A three-step chromatographic procedure using orthogonal separation mechanisms (size-exclusion, cation-exchange and ion-pairing reversed phase) was developed to purify three low molecular weight selenospecies, including the major compound, from the aqueous extract of monkeypot (Lecythis minor) nuts. The following reversed-phase nanoHPLC-electrospray Q-TOF-MS/MS allowed the formal standardless identification of selenocystathionine and two isoforms of γ-glutamyl-selenocystathionine. This is the first MS and MS/MS-based formal evidence of the presence of these compounds in a biological sample.

Original languageEnglish
Pages (from-to)439-449
Number of pages11
JournalAnalyst
Volume132
Issue number5
DOIs
Publication statusPublished - 8 May 2007
Externally publishedYes

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ASJC Scopus subject areas

  • Analytical Chemistry

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