Sr2+ can become incorporated into an agonist-sensitive, cytoplasmic Ca2+ store in a cell line derived from the equine sweat gland epithelium

W. H. Ko, J. D. Pediani, Douglas Bovell, S. M. Wilson

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We have explored the properties of a Ca2+-dependent cell-signalling pathway that becomes active when cultured equine sweat gland cells are stimulated with ATP. The ATP-regulated, Ca2+-influx pathway allowed Sr2+ to enter the cytoplasm but permitted only a minimal influx of Ba2+. Experiments in which cells were repeatedly stimulated with ATP suggested that Sr2+, but not Ba2+, could become incorporated into the agonist-sensitive, cytoplasmic Ca2+ store. Further evidence for this was provided by experiments using ionomycin, a Ca2+ ionophore which has no affinity for Sr2+.

Original languageEnglish
Pages (from-to)804-808
Number of pages5
JournalExperientia
Volume51
Issue number8
DOIs
Publication statusPublished - Aug 1995
Externally publishedYes

Fingerprint

Sweat Glands
Horses
Epithelium
Adenosine Triphosphate
Cells
Cell Line
Cell signaling
Ionomycin
Ionophores
Cytoplasm
Experiments

Keywords

  • Ca mobilisation
  • epithelial cells
  • Fura-2
  • horse
  • ionomycin
  • nucleotide receptors
  • purinergic receptors
  • sweat gland

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Sr2+ can become incorporated into an agonist-sensitive, cytoplasmic Ca2+ store in a cell line derived from the equine sweat gland epithelium. / Ko, W. H.; Pediani, J. D.; Bovell, Douglas; Wilson, S. M.

In: Experientia, Vol. 51, No. 8, 08.1995, p. 804-808.

Research output: Contribution to journalArticle

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