Spontaneous expression of the c-sis gene and release of a platelet-derived growth factorlike molecule by human alveolar macrophages

J. F. Mornex, Y. Martinet, K. Yamauchi, P. B. Bitterman, G. R. Grotendorst, A. Chytil-Weir, Ronald Crystal

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Alveolar macrophages from normal individuals and patients with interstitial lung diseases spontaneously expressed a 4.2-kilobase mRNA complementary to the c-sis gene, a proto-oncogene coding for one of the chains of platelet-derived growth factor (PDGF). Concomitantly, these cells released a mediator with the properties of PDGF, including: (a) chemotactic factor for smooth muscle cells whose activity was resistant to heat and acid, but sensitive to reduction; (b) mitogenic (competence) activity for fibroblasts; (c) ability to compete with PDGF for its receptor; and (d) precipitated by an anti-PDGF antibody. While blood monocytes did not contain c-sis mRNA transcripts, monocytes matured in vitro expressed c-sis, consistent with the concept that expression of c-sis occurs during the differentiation of monocytes into alveolar macrophages. Together with the known actions of PDGF, these observations suggest that the c-sis proto-oncogene and its PDGF product are part of the armamentarium available to the alveolar macrophages for normal lung defense and participation in lung inflammation.

Original languageEnglish
Pages (from-to)61-66
Number of pages6
JournalJournal of Clinical Investigation
Volume78
Issue number1
DOIs
Publication statusPublished - 1 Jan 1986
Externally publishedYes

Fingerprint

sis Genes
Platelet-Derived Growth Factor
Alveolar Macrophages
Blood Platelets
Growth
Monocytes
Platelet-Derived Growth Factor Receptors
Messenger RNA
Aptitude
Proto-Oncogenes
Chemotactic Factors
Interstitial Lung Diseases
Mental Competency
Smooth Muscle Myocytes
Pneumonia
Fibroblasts
Hot Temperature
Lung
Acids
Antibodies

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Spontaneous expression of the c-sis gene and release of a platelet-derived growth factorlike molecule by human alveolar macrophages. / Mornex, J. F.; Martinet, Y.; Yamauchi, K.; Bitterman, P. B.; Grotendorst, G. R.; Chytil-Weir, A.; Crystal, Ronald.

In: Journal of Clinical Investigation, Vol. 78, No. 1, 01.01.1986, p. 61-66.

Research output: Contribution to journalArticle

Mornex, J. F. ; Martinet, Y. ; Yamauchi, K. ; Bitterman, P. B. ; Grotendorst, G. R. ; Chytil-Weir, A. ; Crystal, Ronald. / Spontaneous expression of the c-sis gene and release of a platelet-derived growth factorlike molecule by human alveolar macrophages. In: Journal of Clinical Investigation. 1986 ; Vol. 78, No. 1. pp. 61-66.
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