Specific fiber deficits in sensorimotor diabetic polyneuropathy correspond to cytotoxicity against neuroblastoma cells of sera from patients with diabetes

Gary L. Pittenger, Rayaz Malik, Niculina Burcus, Andrew J. Boulton, Aaron I. Vinik

Research output: Contribution to journalArticle

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Abstract

OBJECTIVE - Neuropathy is the most common complication of diabetes, and toxic serum factors may contribute to its genesis. RESEARCH DESIGN AND METHODS - We assessed neurotoxicity in the serum of 39 diabetic patients and correlated it with clinical measures of somatic and autonomic nerve fiber damage. Sera were applied to N1E-115 and VSC4.1 neuroblastoma cells in vitro as models of sensory/autonomic (S/A) and motor neurons, respectively. Neurotoxicity was measured as either complete or near-complete cell death (highly toxic), inhibited cell growth (moderately toxic), or normal cell proliferation (nontoxic) compared with pooled human serum controls during culture over 4 days. RESULTS - There was an inverse correlation between neurotoxicity and vibration perception threshold (P < 0.01). Age (P < 0.02), duration of diabetes (P < 0.02), and HbA(1c) (P < 0.03) correlated with neurotoxicity, suggesting that glycation may contribute to cytotoxicity in this model. S/A neurotoxicity occurred more frequently in the sera of patients with type 1 (19 of 25) than type 2 (5 of 14) diabetes (P < 0.02). None of the sera from either type 1 or type 2 diabetic patients displayed neurotoxicity on VSC4.1 cells, whereas sera from patients with motor neuropathy were highly toxic. CONCLUSIONS - These studies indicate that there is a relationship between the specific nerve fiber dysfunction in the patient and the type of neuronal cell killed, not only for diabetic neuropathy but also for known forms of autoimmune neuropathies. Such toxic factors may contribute to diabetic neuropathy by acting in concert with hyperglycemia to damage sensory/autonomic neurons.

Original languageEnglish
Pages (from-to)1839-1844
Number of pages6
JournalDiabetes Care
Volume22
Issue number11
DOIs
Publication statusPublished - Nov 1999
Externally publishedYes

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Diabetic Neuropathies
Neuroblastoma
Poisons
Serum
Sensory Receptor Cells
Nerve Fibers
Autonomic Pathways
Motor Neurons
Diabetes Complications
Vibration
Hyperglycemia
Cell Death
Research Design
Cell Proliferation
Growth

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Specific fiber deficits in sensorimotor diabetic polyneuropathy correspond to cytotoxicity against neuroblastoma cells of sera from patients with diabetes. / Pittenger, Gary L.; Malik, Rayaz; Burcus, Niculina; Boulton, Andrew J.; Vinik, Aaron I.

In: Diabetes Care, Vol. 22, No. 11, 11.1999, p. 1839-1844.

Research output: Contribution to journalArticle

Pittenger, Gary L. ; Malik, Rayaz ; Burcus, Niculina ; Boulton, Andrew J. ; Vinik, Aaron I. / Specific fiber deficits in sensorimotor diabetic polyneuropathy correspond to cytotoxicity against neuroblastoma cells of sera from patients with diabetes. In: Diabetes Care. 1999 ; Vol. 22, No. 11. pp. 1839-1844.
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