Abstract
The LIM/homeodomain transcription factor Lim-1 has been shown to play an essential role in early embryonic patterning during vertebrate development. Here we report the spatial and temporal expression patterns of Lim-1 during retinal development as detected by immunohistochemistry using a specific anti-Lim-1 antibody. By double-immunostaining, we have demonstrated for the first time that Lim-1 is exclusively expressed within the horizontal cell type in the adult retina. In the developing mouse retina, Lim-1 commences its expression in migratory horizontal cell precursors streaming toward the future horizontal cell layer in the ventricular zone. Moreover, its expression during retinogenesis is spatially and temporally coincident with that of the calcium-binding protein calbindin D-28k in horizontal cells. These data together suggest a possible role for Lim-1 in terminal differentiation and maintenance of horizontal cells, and that Lim-1 can serve as a specific molecular marker for the study of horizontal cell specification. (C) 2000 Wiley-Liss, Inc.
| Original language | English |
|---|---|
| Pages (from-to) | 320-325 |
| Number of pages | 6 |
| Journal | Developmental Dynamics |
| Volume | 217 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2000 |
| Externally published | Yes |
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Keywords
- Homeodomain
- Horizontal cell
- Lim-1
- Retinogenesis
- Transcription factor
ASJC Scopus subject areas
- Developmental Biology
- Cell Biology
Cite this
Specific expression of the LIM/homeodomain protein Lim-1 in horizontal cells during retinogenesis. / Liu, Wei; Wang, Jian Hua; Xiang, Mengqing.
In: Developmental Dynamics, Vol. 217, No. 3, 2000, p. 320-325.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Specific expression of the LIM/homeodomain protein Lim-1 in horizontal cells during retinogenesis
AU - Liu, Wei
AU - Wang, Jian Hua
AU - Xiang, Mengqing
PY - 2000
Y1 - 2000
N2 - The LIM/homeodomain transcription factor Lim-1 has been shown to play an essential role in early embryonic patterning during vertebrate development. Here we report the spatial and temporal expression patterns of Lim-1 during retinal development as detected by immunohistochemistry using a specific anti-Lim-1 antibody. By double-immunostaining, we have demonstrated for the first time that Lim-1 is exclusively expressed within the horizontal cell type in the adult retina. In the developing mouse retina, Lim-1 commences its expression in migratory horizontal cell precursors streaming toward the future horizontal cell layer in the ventricular zone. Moreover, its expression during retinogenesis is spatially and temporally coincident with that of the calcium-binding protein calbindin D-28k in horizontal cells. These data together suggest a possible role for Lim-1 in terminal differentiation and maintenance of horizontal cells, and that Lim-1 can serve as a specific molecular marker for the study of horizontal cell specification. (C) 2000 Wiley-Liss, Inc.
AB - The LIM/homeodomain transcription factor Lim-1 has been shown to play an essential role in early embryonic patterning during vertebrate development. Here we report the spatial and temporal expression patterns of Lim-1 during retinal development as detected by immunohistochemistry using a specific anti-Lim-1 antibody. By double-immunostaining, we have demonstrated for the first time that Lim-1 is exclusively expressed within the horizontal cell type in the adult retina. In the developing mouse retina, Lim-1 commences its expression in migratory horizontal cell precursors streaming toward the future horizontal cell layer in the ventricular zone. Moreover, its expression during retinogenesis is spatially and temporally coincident with that of the calcium-binding protein calbindin D-28k in horizontal cells. These data together suggest a possible role for Lim-1 in terminal differentiation and maintenance of horizontal cells, and that Lim-1 can serve as a specific molecular marker for the study of horizontal cell specification. (C) 2000 Wiley-Liss, Inc.
KW - Homeodomain
KW - Horizontal cell
KW - Lim-1
KW - Retinogenesis
KW - Transcription factor
UR - http://www.scopus.com/inward/record.url?scp=0034008333&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034008333&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1097-0177(200003)217:3<320::AID-DVDY10>3.0.CO;2-F
DO - 10.1002/(SICI)1097-0177(200003)217:3<320::AID-DVDY10>3.0.CO;2-F
M3 - Article
C2 - 10741426
AN - SCOPUS:0034008333
VL - 217
SP - 320
EP - 325
JO - Developmental Dynamics
JF - Developmental Dynamics
SN - 1058-8388
IS - 3
ER -