Sodium dodecyl sulfate agarose gel electropheresis and electroelution of high molecular weight human salivary mucin

Matthias Gabriel, Andrej Zentner

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The aim of this study was to test the applicability of sodium dodecyl sulfate (SDS) agarose gel electrophoresis, electroelution and electrophoretic filtration as methods of separation, detection, and purification of high molecular weight human salivary mucin. SDS agarose gel electrophoresis of whole saliva and mucin prepared by density gradient ultracentrifugation revealed bands with molecular weights in excess of 450 kDa and between 1.6×106 and 2×106 Da. Electroelution of material from gel and subsequent electrophoresis resulted in highly purified high molecular weight material. Electrophoretic filtration, a method of collecting the material remaining from whole saliva in the slot after penetration of low molecular weight constituents into SDS polyacrylamide gel, failed to produce pure high molecular weight material. It is concluded that SDS agarose gel electrophoresis and electroelution are suitable methods for studying high molecular weight salivary mucin glycoproteins.

Original languageEnglish
Pages (from-to)76-78
Number of pages3
JournalClinical Oral Investigations
Volume9
Issue number4
DOIs
Publication statusPublished - Dec 2005
Externally publishedYes

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Keywords

  • Agarose
  • Electroelution
  • Electrophoresis
  • Mucin
  • Saliva

ASJC Scopus subject areas

  • Dentistry(all)
  • Oral Surgery
  • Periodontics
  • Orthodontics

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