Abstract
The aim of this study was to test the applicability of sodium dodecyl sulfate (SDS) agarose gel electrophoresis, electroelution and electrophoretic filtration as methods of separation, detection, and purification of high molecular weight human salivary mucin. SDS agarose gel electrophoresis of whole saliva and mucin prepared by density gradient ultracentrifugation revealed bands with molecular weights in excess of 450 kDa and between 1.6×106 and 2×106 Da. Electroelution of material from gel and subsequent electrophoresis resulted in highly purified high molecular weight material. Electrophoretic filtration, a method of collecting the material remaining from whole saliva in the slot after penetration of low molecular weight constituents into SDS polyacrylamide gel, failed to produce pure high molecular weight material. It is concluded that SDS agarose gel electrophoresis and electroelution are suitable methods for studying high molecular weight salivary mucin glycoproteins.
| Original language | English |
|---|---|
| Pages (from-to) | 76-78 |
| Number of pages | 3 |
| Journal | Clinical Oral Investigations |
| Volume | 9 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Dec 2005 |
| Externally published | Yes |
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Keywords
- Agarose
- Electroelution
- Electrophoresis
- Mucin
- Saliva
ASJC Scopus subject areas
- Dentistry(all)
- Oral Surgery
- Periodontics
- Orthodontics
Cite this
Sodium dodecyl sulfate agarose gel electropheresis and electroelution of high molecular weight human salivary mucin. / Gabriel, Matthias; Zentner, Andrej.
In: Clinical Oral Investigations, Vol. 9, No. 4, 12.2005, p. 76-78.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Sodium dodecyl sulfate agarose gel electropheresis and electroelution of high molecular weight human salivary mucin
AU - Gabriel, Matthias
AU - Zentner, Andrej
PY - 2005/12
Y1 - 2005/12
N2 - The aim of this study was to test the applicability of sodium dodecyl sulfate (SDS) agarose gel electrophoresis, electroelution and electrophoretic filtration as methods of separation, detection, and purification of high molecular weight human salivary mucin. SDS agarose gel electrophoresis of whole saliva and mucin prepared by density gradient ultracentrifugation revealed bands with molecular weights in excess of 450 kDa and between 1.6×106 and 2×106 Da. Electroelution of material from gel and subsequent electrophoresis resulted in highly purified high molecular weight material. Electrophoretic filtration, a method of collecting the material remaining from whole saliva in the slot after penetration of low molecular weight constituents into SDS polyacrylamide gel, failed to produce pure high molecular weight material. It is concluded that SDS agarose gel electrophoresis and electroelution are suitable methods for studying high molecular weight salivary mucin glycoproteins.
AB - The aim of this study was to test the applicability of sodium dodecyl sulfate (SDS) agarose gel electrophoresis, electroelution and electrophoretic filtration as methods of separation, detection, and purification of high molecular weight human salivary mucin. SDS agarose gel electrophoresis of whole saliva and mucin prepared by density gradient ultracentrifugation revealed bands with molecular weights in excess of 450 kDa and between 1.6×106 and 2×106 Da. Electroelution of material from gel and subsequent electrophoresis resulted in highly purified high molecular weight material. Electrophoretic filtration, a method of collecting the material remaining from whole saliva in the slot after penetration of low molecular weight constituents into SDS polyacrylamide gel, failed to produce pure high molecular weight material. It is concluded that SDS agarose gel electrophoresis and electroelution are suitable methods for studying high molecular weight salivary mucin glycoproteins.
KW - Agarose
KW - Electroelution
KW - Electrophoresis
KW - Mucin
KW - Saliva
UR - http://www.scopus.com/inward/record.url?scp=31044454268&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=31044454268&partnerID=8YFLogxK
U2 - 10.1007/s00784-005-0007-2
DO - 10.1007/s00784-005-0007-2
M3 - Article
VL - 9
SP - 76
EP - 78
JO - Clinical Oral Investigations
JF - Clinical Oral Investigations
SN - 1432-6981
IS - 4
ER -