Smoking-induced CXCL14 expression in the human airway epithelium links chronic obstructive pulmonary disease to lung cancer

Renat Shaykhiev, Rachel Sackrowitz, Tomoya Fukui, Wu Lin Zuo, Ion Wa Chao, Yael Strulovici-Barel, Robert J. Downey, Ronald Crystal

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

CXCL14, a recently described epithelial cytokine, plays putative multiple roles in inflammation and carcinogenesis. In the context that chronic obstructive pulmonary disease (COPD) and lung cancer are both smoking-related disorders associated with airway epithelial disorder and inflammation, we hypothesized that the airway epithelium responds to cigarette smoking with altered CXCL14 gene expression, contributing to the disease-relevant phenotype. Using genome-wide microarrays with subsequent immunohistochemical analysis, the data demonstrate that the expression of CXCL14 is up-regulated in the airway epithelium of healthy smokers and further increased in COPD smokers, especially within hyperplastic/metaplastic lesions, in association with multiple genes relevant to epithelial structural integrity and cancer. In vitro experiments revealed that the expression of CXCL14 is induced in the differentiated airway epithelium by cigarette smoke extract, and that epidermal growth factor mediates CXCL14 upregulation in the airway epithelium through its effects on the basal stem/progenitor cell population. Analyses of two independent lung cancer cohorts revealed a dramatic up-regulation of CXCL14 expression in adenocarcinoma and squamous-cell carcinoma. High expression of the COPD-associated CXCL14-correlating cluster of geneswas linked in lung adenocarcinoma with poor survival. These data suggest that the smoking-induced expression of CXCL14 in the airway epithelium represents a novel potential molecular link between smoking-associated airway epithelial injury, COPD, and lung cancer.

Original languageEnglish
Pages (from-to)418-425
Number of pages8
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume49
Issue number3
DOIs
Publication statusPublished - 1 Sep 2013
Externally publishedYes

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Pulmonary diseases
Chronic Obstructive Pulmonary Disease
Lung Neoplasms
Epithelium
Smoking
Up-Regulation
Stem Cells
Tobacco Products
Inflammation
Genes
Epidermal Growth Factor
Smoke
Squamous Cell Carcinoma
Carcinogenesis
Adenocarcinoma
Structural integrity
Microarrays
Stem cells
Genome
Gene expression

Keywords

  • Airway epithelium
  • COPD
  • CXCL14
  • Lung cancer
  • Smoking

ASJC Scopus subject areas

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology

Cite this

Smoking-induced CXCL14 expression in the human airway epithelium links chronic obstructive pulmonary disease to lung cancer. / Shaykhiev, Renat; Sackrowitz, Rachel; Fukui, Tomoya; Zuo, Wu Lin; Chao, Ion Wa; Strulovici-Barel, Yael; Downey, Robert J.; Crystal, Ronald.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 49, No. 3, 01.09.2013, p. 418-425.

Research output: Contribution to journalArticle

Shaykhiev, Renat ; Sackrowitz, Rachel ; Fukui, Tomoya ; Zuo, Wu Lin ; Chao, Ion Wa ; Strulovici-Barel, Yael ; Downey, Robert J. ; Crystal, Ronald. / Smoking-induced CXCL14 expression in the human airway epithelium links chronic obstructive pulmonary disease to lung cancer. In: American Journal of Respiratory Cell and Molecular Biology. 2013 ; Vol. 49, No. 3. pp. 418-425.
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