SIRT1 limits adipocyte hyperplasia through c-Myc inhibition

Houari Abdesselem, Aisha Madani, Ahmad Hani, Muna Al-Noubi, Neha Goswami, Hisham Ben Hamidane, Anja Billing, Jennifer Pasquier, Michael S. Bonkowski, Najeeb Halabi, Rajaa Dalloul, Mohamed Z. Sheriff, Nasrin Mesaeli, Mohamed ElRayess, David A. Sinclair, Johannes Graumann, Nayef Mazloum

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The expansion of fat mass in the obese state is due to increased adipocyte hypertrophy and hyperplasia. The molecular mechanism that drives adipocyte hyperplasia remains unknown. The NAD+-dependent protein deacetylase sirtuin 1 (SIRT1), a key regulator of mammalian metabolism, maintains proper metabolic functions in many tissues, counteracting obesity. Here we report that differentiated adipocytes are hyperplastic when SIRT1 is knocked down stably in mouse 3T3-L1 preadipocytes. This phenotype is associated with dysregulated adipocyte metabolism and enhanced inflammation. We also demonstrate that SIRT1 is a key regulator of proliferation in preadipocytes. Quantitative proteomics reveal that the c-Myc pathway is altered to drive enhanced proliferation in SIRT1-silenced 3T3-L1 cells. Moreover, c-Myc is hyperacetylated, levels of p27 are reduced, and cyclin-dependent kinase 2 (CDK2) is activated upon SIRT1 reduction. Remarkably, differentiating SIRT1-silenced preadipocytes exhibit enhanced mitotic clonal expansion accompanied by reduced levels of p27 as well as elevated levels of CCAAT/enhancer-binding protein β (C/EBPβ) and c-Myc, which is also hyperacetylated. c-Myc activation and enhanced proliferation phenotype are also found to be SIRT1- dependent in proliferating mouse embryonic fibroblasts and differentiating human SW872 preadipocytes. Reducing both SIRT1 and c-Myc expression in 3T3-L1 cells simultaneously does not induce the adipocyte hyperplasia phenotype, confirming that SIRT1 controls adipocyte hyperplasia through c-Myc regulation. A better understanding of the molecular mechanisms of adipocyte hyperplasia will open new avenues toward understanding obesity.

Original languageEnglish
Pages (from-to)2119-2135
Number of pages17
JournalJournal of Biological Chemistry
Volume291
Issue number5
DOIs
Publication statusPublished - 29 Jan 2016

Fingerprint

Sirtuin 1
Adipocytes
Hyperplasia
3T3-L1 Cells
Phenotype
Metabolism
Obesity
Cyclin-Dependent Kinase 2
CCAAT-Enhancer-Binding Proteins
Fibroblasts
NAD
Proteomics
Hypertrophy
Chemical activation
Fats

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

SIRT1 limits adipocyte hyperplasia through c-Myc inhibition. / Abdesselem, Houari; Madani, Aisha; Hani, Ahmad; Al-Noubi, Muna; Goswami, Neha; Ben Hamidane, Hisham; Billing, Anja; Pasquier, Jennifer; Bonkowski, Michael S.; Halabi, Najeeb; Dalloul, Rajaa; Sheriff, Mohamed Z.; Mesaeli, Nasrin; ElRayess, Mohamed; Sinclair, David A.; Graumann, Johannes; Mazloum, Nayef.

In: Journal of Biological Chemistry, Vol. 291, No. 5, 29.01.2016, p. 2119-2135.

Research output: Contribution to journalArticle

Abdesselem, Houari ; Madani, Aisha ; Hani, Ahmad ; Al-Noubi, Muna ; Goswami, Neha ; Ben Hamidane, Hisham ; Billing, Anja ; Pasquier, Jennifer ; Bonkowski, Michael S. ; Halabi, Najeeb ; Dalloul, Rajaa ; Sheriff, Mohamed Z. ; Mesaeli, Nasrin ; ElRayess, Mohamed ; Sinclair, David A. ; Graumann, Johannes ; Mazloum, Nayef. / SIRT1 limits adipocyte hyperplasia through c-Myc inhibition. In: Journal of Biological Chemistry. 2016 ; Vol. 291, No. 5. pp. 2119-2135.
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AU - Hani, Ahmad

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AU - Goswami, Neha

AU - Ben Hamidane, Hisham

AU - Billing, Anja

AU - Pasquier, Jennifer

AU - Bonkowski, Michael S.

AU - Halabi, Najeeb

AU - Dalloul, Rajaa

AU - Sheriff, Mohamed Z.

AU - Mesaeli, Nasrin

AU - ElRayess, Mohamed

AU - Sinclair, David A.

AU - Graumann, Johannes

AU - Mazloum, Nayef

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