Simultaneous measurement and modulation of multiple physiological parameters in the isolated heart using optical techniques

Peter Lee, Ping Yan, Paul Ewart, Peter Kohl, Leslie M. Loew, Christian Bollensdorff

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Whole-heart multi-parametric optical mapping has provided valuable insight into the interplay of electrophysiological parameters, and this technology will continue to thrive as dyes are improved and technical solutions for imaging become simpler and cheaper. Here, we show the advantage of using improved 2nd-generation voltage dyes, provide a simple solution to panoramic multi-parametric mapping, and illustrate the application of flash photolysis of caged compounds for studies in the whole heart. For proof of principle, we used the isolated rat whole-heart model. After characterising the blue and green isosbestic points of di-4-ANBDQBS and di-4-ANBDQPQ, respectively, two voltage and calcium mapping systems are described. With two newly custom-made multi-band optical filters, (1) di-4-ANBDQBS and fluo-4 and (2) di-4-ANBDQPQ and rhod-2 mapping are demonstrated. Furthermore, we demonstrate three-parameter mapping using di-4-ANBDQPQ, rhod-2 and NADH. Using off-the-shelf optics and the di-4- ANBDQPQ and rhod-2 combination, we demonstrate panoramic multi-parametric mapping, affording a 360° spatiotemporal record of activity. Finally, local optical perturbation of calcium dynamics in the whole heart is demonstrated using the caged compound, o-nitrophenyl ethylene glycol tetraacetic acid (NP-EGTA), with an ultraviolet light-emitting diode (LED). Calcium maps (heart loaded with di-4-ANBDQPQ and rhod-2) demonstrate successful NP-EGTA loading and local flash photolysis. All imaging systems were built using only a single camera. In conclusion, using novel 2nd-generation voltage dyes, we developed scalable techniques for multi-parametric optical mapping of the whole heart from one point of view and panoramically. In addition to these parameter imaging approaches, we show that it is possible to use caged compounds and ultraviolet LEDs to locally perturb electrophysiological parameters in the whole heart.

Original languageEnglish
Pages (from-to)403-414
Number of pages12
JournalPflugers Archiv European Journal of Physiology
Volume464
Issue number4
DOIs
Publication statusPublished - 1 Oct 2012
Externally publishedYes

Fingerprint

Modulation
Coloring Agents
Photolysis
Egtazic Acid
Calcium
Light emitting diodes
Electric potential
Imaging techniques
Optical filters
Ultraviolet Rays
Imaging systems
NAD
Rats
Optics
Cameras
Technology
rhod-2
1-(4-sulfanatobutyl)-4-(beta-(2-(di-n-butylamino)-6-naphthyl)butadienyl)quinolinium betaine

Keywords

  • Arrhythmia
  • Electrophysiology
  • Flash photolysis
  • Multi-Parametric
  • Optical mapping

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)

Cite this

Simultaneous measurement and modulation of multiple physiological parameters in the isolated heart using optical techniques. / Lee, Peter; Yan, Ping; Ewart, Paul; Kohl, Peter; Loew, Leslie M.; Bollensdorff, Christian.

In: Pflugers Archiv European Journal of Physiology, Vol. 464, No. 4, 01.10.2012, p. 403-414.

Research output: Contribution to journalArticle

Lee, Peter ; Yan, Ping ; Ewart, Paul ; Kohl, Peter ; Loew, Leslie M. ; Bollensdorff, Christian. / Simultaneous measurement and modulation of multiple physiological parameters in the isolated heart using optical techniques. In: Pflugers Archiv European Journal of Physiology. 2012 ; Vol. 464, No. 4. pp. 403-414.
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