Simultaneous electrospin-electrosprayed biocomposite nanofibrous scaffolds for bone tissue regeneration

Lijo Francis, J. Venugopal, Molamma P. Prabhakaran, V. Thavasi, E. Marsano, S. Ramakrishna

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Currently, the application of nanotechnology in bone tissue regeneration is a challenge for the fabrication of novel bioartificial bone grafts. These nanostructures are capable of mimicking natural extracellular matrix with effective mineralization for successful regeneration of damaged tissues. The simultaneous electrospraying of nanohydroxyapatite (HA) on electrospun polymeric nanofibrous scaffolds might be more promising for bone tissue regeneration. In the current study, nanofibrous scaffolds of gelatin (Gel), Gel/HA (4:1 blend), Gel/HA (2:1 blend) and Gel/HA (electrospin-electrospray) were fabricated for this purpose. The morphology, chemical and mechanical stability of nanofibres were evaluated by means of field emission scanning electron microscopy (FESEM), Fourier transform infrared spectroscopy and with a universal tensile machine, respectively. The in vitro biocompatibility of different nanofibrous scaffolds was determined by culturing human foetal osteoblasts and investigating the proliferation, alkaline phosphatase (ALP) activity and mineralization of cells. The results of cell proliferation, ALP activity and FESEM studies revealed that the combination of electrospinning of gelatin and electrospraying of HA yielded biocomposite nanofibrous scaffolds with enhanced performances in terms of better cell proliferation, increased ALP activity and enhanced mineralization, making them potential substrates for bone tissue regeneration.

Original languageEnglish
Pages (from-to)4100-4109
Number of pages10
JournalActa Biomaterialia
Volume6
Issue number10
DOIs
Publication statusPublished - Oct 2010
Externally publishedYes

Fingerprint

Tissue regeneration
Bone Regeneration
Gelatin
Scaffolds
Phosphatases
Bone
Bone and Bones
Cell proliferation
Alkaline Phosphatase
Scaffolds (biology)
Field emission
Electron Scanning Microscopy
Scanning electron microscopy
Cell Proliferation
Mechanical stability
Osteoblasts
Chemical stability
Electrospinning
Nanofibers
Biocompatibility

Keywords

  • Electrospinning
  • Electrospraying
  • Hydroxyapatite
  • Mineralization
  • Osteoblasts

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Molecular Biology

Cite this

Simultaneous electrospin-electrosprayed biocomposite nanofibrous scaffolds for bone tissue regeneration. / Francis, Lijo; Venugopal, J.; Prabhakaran, Molamma P.; Thavasi, V.; Marsano, E.; Ramakrishna, S.

In: Acta Biomaterialia, Vol. 6, No. 10, 10.2010, p. 4100-4109.

Research output: Contribution to journalArticle

Francis, Lijo ; Venugopal, J. ; Prabhakaran, Molamma P. ; Thavasi, V. ; Marsano, E. ; Ramakrishna, S. / Simultaneous electrospin-electrosprayed biocomposite nanofibrous scaffolds for bone tissue regeneration. In: Acta Biomaterialia. 2010 ; Vol. 6, No. 10. pp. 4100-4109.
@article{34fde3a6da864fa9a5dd36d5dead2b58,
title = "Simultaneous electrospin-electrosprayed biocomposite nanofibrous scaffolds for bone tissue regeneration",
abstract = "Currently, the application of nanotechnology in bone tissue regeneration is a challenge for the fabrication of novel bioartificial bone grafts. These nanostructures are capable of mimicking natural extracellular matrix with effective mineralization for successful regeneration of damaged tissues. The simultaneous electrospraying of nanohydroxyapatite (HA) on electrospun polymeric nanofibrous scaffolds might be more promising for bone tissue regeneration. In the current study, nanofibrous scaffolds of gelatin (Gel), Gel/HA (4:1 blend), Gel/HA (2:1 blend) and Gel/HA (electrospin-electrospray) were fabricated for this purpose. The morphology, chemical and mechanical stability of nanofibres were evaluated by means of field emission scanning electron microscopy (FESEM), Fourier transform infrared spectroscopy and with a universal tensile machine, respectively. The in vitro biocompatibility of different nanofibrous scaffolds was determined by culturing human foetal osteoblasts and investigating the proliferation, alkaline phosphatase (ALP) activity and mineralization of cells. The results of cell proliferation, ALP activity and FESEM studies revealed that the combination of electrospinning of gelatin and electrospraying of HA yielded biocomposite nanofibrous scaffolds with enhanced performances in terms of better cell proliferation, increased ALP activity and enhanced mineralization, making them potential substrates for bone tissue regeneration.",
keywords = "Electrospinning, Electrospraying, Hydroxyapatite, Mineralization, Osteoblasts",
author = "Lijo Francis and J. Venugopal and Prabhakaran, {Molamma P.} and V. Thavasi and E. Marsano and S. Ramakrishna",
year = "2010",
month = "10",
doi = "10.1016/j.actbio.2010.05.001",
language = "English",
volume = "6",
pages = "4100--4109",
journal = "Acta Biomaterialia",
issn = "1742-7061",
publisher = "Elsevier BV",
number = "10",

}

TY - JOUR

T1 - Simultaneous electrospin-electrosprayed biocomposite nanofibrous scaffolds for bone tissue regeneration

AU - Francis, Lijo

AU - Venugopal, J.

AU - Prabhakaran, Molamma P.

AU - Thavasi, V.

AU - Marsano, E.

AU - Ramakrishna, S.

PY - 2010/10

Y1 - 2010/10

N2 - Currently, the application of nanotechnology in bone tissue regeneration is a challenge for the fabrication of novel bioartificial bone grafts. These nanostructures are capable of mimicking natural extracellular matrix with effective mineralization for successful regeneration of damaged tissues. The simultaneous electrospraying of nanohydroxyapatite (HA) on electrospun polymeric nanofibrous scaffolds might be more promising for bone tissue regeneration. In the current study, nanofibrous scaffolds of gelatin (Gel), Gel/HA (4:1 blend), Gel/HA (2:1 blend) and Gel/HA (electrospin-electrospray) were fabricated for this purpose. The morphology, chemical and mechanical stability of nanofibres were evaluated by means of field emission scanning electron microscopy (FESEM), Fourier transform infrared spectroscopy and with a universal tensile machine, respectively. The in vitro biocompatibility of different nanofibrous scaffolds was determined by culturing human foetal osteoblasts and investigating the proliferation, alkaline phosphatase (ALP) activity and mineralization of cells. The results of cell proliferation, ALP activity and FESEM studies revealed that the combination of electrospinning of gelatin and electrospraying of HA yielded biocomposite nanofibrous scaffolds with enhanced performances in terms of better cell proliferation, increased ALP activity and enhanced mineralization, making them potential substrates for bone tissue regeneration.

AB - Currently, the application of nanotechnology in bone tissue regeneration is a challenge for the fabrication of novel bioartificial bone grafts. These nanostructures are capable of mimicking natural extracellular matrix with effective mineralization for successful regeneration of damaged tissues. The simultaneous electrospraying of nanohydroxyapatite (HA) on electrospun polymeric nanofibrous scaffolds might be more promising for bone tissue regeneration. In the current study, nanofibrous scaffolds of gelatin (Gel), Gel/HA (4:1 blend), Gel/HA (2:1 blend) and Gel/HA (electrospin-electrospray) were fabricated for this purpose. The morphology, chemical and mechanical stability of nanofibres were evaluated by means of field emission scanning electron microscopy (FESEM), Fourier transform infrared spectroscopy and with a universal tensile machine, respectively. The in vitro biocompatibility of different nanofibrous scaffolds was determined by culturing human foetal osteoblasts and investigating the proliferation, alkaline phosphatase (ALP) activity and mineralization of cells. The results of cell proliferation, ALP activity and FESEM studies revealed that the combination of electrospinning of gelatin and electrospraying of HA yielded biocomposite nanofibrous scaffolds with enhanced performances in terms of better cell proliferation, increased ALP activity and enhanced mineralization, making them potential substrates for bone tissue regeneration.

KW - Electrospinning

KW - Electrospraying

KW - Hydroxyapatite

KW - Mineralization

KW - Osteoblasts

UR - http://www.scopus.com/inward/record.url?scp=77956189952&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77956189952&partnerID=8YFLogxK

U2 - 10.1016/j.actbio.2010.05.001

DO - 10.1016/j.actbio.2010.05.001

M3 - Article

VL - 6

SP - 4100

EP - 4109

JO - Acta Biomaterialia

JF - Acta Biomaterialia

SN - 1742-7061

IS - 10

ER -