Similarity of gene expression patterns in human alveolar macrophages in response to Pseudomonas aeruginosa and Burkholderia cepacia

Stefan Worgall, Adriana Heguy, Karsta Luettich, Timothy P. O'Connor, Ben Gary Harvey, Luis E.N. Quadri, Ronald Crystal

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

To determine if differences in the severity of pulmonary infection in cystic fibrosis seen with late isolates of Pseudomonas aeruginosa and Burkholderia cepacia are associated with differences in the initial response of alveolar macrophages (AM) to these pathogens, we assessed gene expression changes in human AM in response to infection with a laboratory strain, early and late clinical isolates of P. aeruginosa, and B. cepacia. Analysis of gene expression changes at the RNA level using oligonucleotide microarrays, following exposure to laboratory P. aeruginosa strain PAK, showed significant (P < 0.01) >2.5-fold upregulation of 42 genes and >2.5-fold downregulation of 45 genes. The majority of the changes in gene expression involved genes as part of inflammatory pathways and signaling systems. Interestingly, similar responses were observed following exposure of AM to early and late clinical isolates of P. aeruginosa, as well as with B. cepacia, suggesting that the more severe clinical outcome of infections with late clinical isolates of P. aeruginosa or with B. cepacia cannot be explained by differences in the early interactions of these organisms with the human AM, as reflected by the similarity of gene expression changes in response to exposure of AM to these pathogens.

Original languageEnglish
Pages (from-to)5262-5268
Number of pages7
JournalInfection and Immunity
Volume73
Issue number8
DOIs
Publication statusPublished - 1 Aug 2005
Externally publishedYes

Fingerprint

Burkholderia cepacia
Alveolar Macrophages
Pseudomonas aeruginosa
Gene Expression
Infection
Genes
Oligonucleotide Array Sequence Analysis
Cystic Fibrosis
Up-Regulation
Down-Regulation
RNA
Lung

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Similarity of gene expression patterns in human alveolar macrophages in response to Pseudomonas aeruginosa and Burkholderia cepacia. / Worgall, Stefan; Heguy, Adriana; Luettich, Karsta; O'Connor, Timothy P.; Harvey, Ben Gary; Quadri, Luis E.N.; Crystal, Ronald.

In: Infection and Immunity, Vol. 73, No. 8, 01.08.2005, p. 5262-5268.

Research output: Contribution to journalArticle

Worgall, Stefan ; Heguy, Adriana ; Luettich, Karsta ; O'Connor, Timothy P. ; Harvey, Ben Gary ; Quadri, Luis E.N. ; Crystal, Ronald. / Similarity of gene expression patterns in human alveolar macrophages in response to Pseudomonas aeruginosa and Burkholderia cepacia. In: Infection and Immunity. 2005 ; Vol. 73, No. 8. pp. 5262-5268.
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