Side Population cells isolated from different tissues share transcriptome signatures and express tissue-specific markers

K. Liadaki, A. T. Kho, D. Sanoudou, J. Schienda, A. Flint, A. H. Beggs, I. S. Kohane, L. M. Kunkel

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Side Population (SP) cells, isolated from murine adult bone marrow (BM) based on the exclusion of the DNA dye Hoechst 33342, exhibit potent hematopoietic stem cell (HSC) activity when compared to Main Population (MP) cells. Furthermore, SP cells derived from murine skeletal muscle exhibit both hematopoietic and myogenic potential in vivo. The multipotential capacity of SP cells isolated from variable tissues is supported by an increasing number of studies. To investigate whether the SP phenotype is associated with a unique transcriptional profile, we characterized gene expression of SP cells isolated from two biologically distinct tissues, bone marrow and muscle. Comparison of SP cells with differentiated MP cells within a tissue revealed that SP cells are in an active transcriptional and translational status and underexpress genes reflecting tissue-specific functions. Direct comparison of gene expression of SP cells isolated from different tissues identified genes common to SP cells as well as genes specific to SP cells within a particular tissue and further define a muscle and bone marrow environment. This study reports gene expression of muscle SP cells, common features and differences between SP cells isolated from muscle and bone marrow, and further identifies common signaling pathways that might regulate SP cell functions.

Original languageEnglish
Pages (from-to)360-374
Number of pages15
JournalExperimental Cell Research
Volume303
Issue number2
DOIs
Publication statusPublished - 15 Feb 2005
Externally publishedYes

Fingerprint

Side-Population Cells
Transcriptome
Bone Marrow
Muscles
Population
Genes
Gene Expression
Hematopoietic Stem Cells
Muscle Cells

Keywords

  • Bone marrow
  • Main Population cells
  • Side Population cells
  • Skeletal muscle

ASJC Scopus subject areas

  • Cell Biology

Cite this

Side Population cells isolated from different tissues share transcriptome signatures and express tissue-specific markers. / Liadaki, K.; Kho, A. T.; Sanoudou, D.; Schienda, J.; Flint, A.; Beggs, A. H.; Kohane, I. S.; Kunkel, L. M.

In: Experimental Cell Research, Vol. 303, No. 2, 15.02.2005, p. 360-374.

Research output: Contribution to journalArticle

Liadaki, K, Kho, AT, Sanoudou, D, Schienda, J, Flint, A, Beggs, AH, Kohane, IS & Kunkel, LM 2005, 'Side Population cells isolated from different tissues share transcriptome signatures and express tissue-specific markers', Experimental Cell Research, vol. 303, no. 2, pp. 360-374. https://doi.org/10.1016/j.yexcr.2004.10.011
Liadaki, K. ; Kho, A. T. ; Sanoudou, D. ; Schienda, J. ; Flint, A. ; Beggs, A. H. ; Kohane, I. S. ; Kunkel, L. M. / Side Population cells isolated from different tissues share transcriptome signatures and express tissue-specific markers. In: Experimental Cell Research. 2005 ; Vol. 303, No. 2. pp. 360-374.
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