Shared and organism-specific host responses to childhood diarrheal diseases revealed by whole blood transcript profiling

Hannah A. DeBerg, Mussaret B. Zaidi, Matthew C. Altman, Prasong Khaenam, Vivian H. Gersuk, Freddy D. Campos, Iza Perez-Martinez, Mario Meza-Segura, Damien J. Chaussabel, Jacques Banchereau, Teresa Estrada-Garcia, Peter S. Linsley

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Globally, diarrheal diseases are a leading cause of death in children under five and disproportionately affect children in developing countries. Children who contract diarrheal diseases are rarely screened to identify the etiologic agent due to time and cost considerations associated with pathogen-specific screening and hence pathogen-directed therapy is uncommon. The development of biomarkers to rapidly identify underlying pathogens could improve treatment options and clinical outcomes in childhood diarrheal diseases. Here, we perform RNA sequencing on blood samples collected from children evaluated in an emergency room setting with diarrheal disease where the pathogen(s) present are known. We determine host response gene signatures specific to Salmonella, Shigella and rotavirus, but not E. coli, infections that distinguish them from each other and from healthy controls. Specifically, we observed differential expression of genes related to chemokine receptors or inflammasome signaling in Shigella cases, such as CCR3, CXCR8, and NLRC4, and interferon response genes, such as IFI44 and OASL, in rotavirus cases. Our findings add insight into the host peripheral immune response to these pathogens, and suggest strategies and limitations for the use host response transcript signatures for diagnosing the etiologic agent of childhood diarrheal diseases.

Original languageEnglish
Article numbere0192082
JournalPLoS One
Volume13
Issue number1
DOIs
Publication statusPublished - 1 Jan 2018

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Pathogens
childhood
Blood
pathogens
blood
organisms
Shigella
Genes
Rotavirus
Emergency rooms
Inflammasomes
RNA Sequence Analysis
Escherichia coli Infections
Salmonella
Chemokine Receptors
Biomarkers
interferons
Developing countries
Escherichia coli
Interferons

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

DeBerg, H. A., Zaidi, M. B., Altman, M. C., Khaenam, P., Gersuk, V. H., Campos, F. D., ... Linsley, P. S. (2018). Shared and organism-specific host responses to childhood diarrheal diseases revealed by whole blood transcript profiling. PLoS One, 13(1), [e0192082]. https://doi.org/10.1371/journal.pone.0192082

Shared and organism-specific host responses to childhood diarrheal diseases revealed by whole blood transcript profiling. / DeBerg, Hannah A.; Zaidi, Mussaret B.; Altman, Matthew C.; Khaenam, Prasong; Gersuk, Vivian H.; Campos, Freddy D.; Perez-Martinez, Iza; Meza-Segura, Mario; Chaussabel, Damien J.; Banchereau, Jacques; Estrada-Garcia, Teresa; Linsley, Peter S.

In: PLoS One, Vol. 13, No. 1, e0192082, 01.01.2018.

Research output: Contribution to journalArticle

DeBerg, HA, Zaidi, MB, Altman, MC, Khaenam, P, Gersuk, VH, Campos, FD, Perez-Martinez, I, Meza-Segura, M, Chaussabel, DJ, Banchereau, J, Estrada-Garcia, T & Linsley, PS 2018, 'Shared and organism-specific host responses to childhood diarrheal diseases revealed by whole blood transcript profiling', PLoS One, vol. 13, no. 1, e0192082. https://doi.org/10.1371/journal.pone.0192082
DeBerg, Hannah A. ; Zaidi, Mussaret B. ; Altman, Matthew C. ; Khaenam, Prasong ; Gersuk, Vivian H. ; Campos, Freddy D. ; Perez-Martinez, Iza ; Meza-Segura, Mario ; Chaussabel, Damien J. ; Banchereau, Jacques ; Estrada-Garcia, Teresa ; Linsley, Peter S. / Shared and organism-specific host responses to childhood diarrheal diseases revealed by whole blood transcript profiling. In: PLoS One. 2018 ; Vol. 13, No. 1.
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